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Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS

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dc.contributor.author Solis, Leonardo
dc.contributor.author Coronel, Jorge
dc.contributor.author Rueda, Daniel
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2019-02-06T14:57:42Z
dc.date.available 2019-02-06T14:57:42Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5500
dc.description.abstract Tuberculosis (TB) control efforts are hampered by a mismatch in diagnostic technology. Lack of adequate early diagnostics and Multi-drug resistant (MDR) detection is a critical problem in control efforts. Alternate and novel diagnostic approaches are required, especially in low-resources settings where they are needed most. The Microscopic Observation Drug Susceptibility (MODS) assay is a cost-effective, highly sensitive, and specific method based on the detection of characteristic cording growth patterns of Mycobacterium tuberculosis (MTB), in microscopic examination of a liquid culture under an inverted microscope. By adding antimicrobials to the wells, MODS also determines antimicrobial susceptibility in both MDR and Extreme Drug Resistant (XDR) tuberculosis. The interpretation of a MODS culture performed in a 24 well plate, requires an extensive inspection over the entire surface to detect TB cords. This process requires significant time and effort from a trained microscopist. We evaluated a lens-free imager system, able to render microscopic images of live specimens, for the proof of principle to be used for MODS culture interpretation. The lens-free imager system is able to digitalize a 24-mm(2) surface with approximately 40X magnification in a single capture. The evaluation of the lens-free imager found that it produced microscopic images that were adequate for MODS interpretation by a human expert. Compared to the average time that takes a microscopist to completely examine a MODS culture sample, the lens free imager notably reduced the time of inspection. Therefore, lens-free imager variants may constitute promising systems to aid in the diagnostics of tuberculosis, by simplifying and reducing the time of inspection and permitting automatization of MODS interpretation. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Tuberculosis
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Tuberculosis en_US
dc.subject Microscopy en_US
dc.subject Time Factors en_US
dc.subject Reproducibility of Results en_US
dc.subject MDR en_US
dc.subject Drug Resistance, Multiple, Bacterial en_US
dc.subject Antitubercular Agents/therapeutic use en_US
dc.subject Equipment Design en_US
dc.subject Predictive Value of Tests en_US
dc.subject Sputum/microbiology en_US
dc.subject Diagnostics en_US
dc.subject ePetri en_US
dc.subject Extensively Drug-Resistant Tuberculosis/diagnosis/drug therapy/microbiology en_US
dc.subject Lens-free imager en_US
dc.subject Lens-free microscopy en_US
dc.subject Microbial Sensitivity Tests/instrumentation en_US
dc.subject Microscopy/instrumentation en_US
dc.subject MODS en_US
dc.subject Multi drug resistance en_US
dc.subject Mycobacterium tuberculosis/drug effects/growth & development en_US
dc.subject Tuberculosis, Multidrug-Resistant/diagnosis/drug therapy/microbiology en_US
dc.subject Tuberculosis, Pulmonary/diagnosis/drug therapy/microbiology en_US
dc.subject Workflow en_US
dc.title Evaluation of a lens-free imager to facilitate tuberculosis diagnostics in MODS en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.tube.2015.12.001
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.07
dc.relation.issn 1873-281X


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