Universidad Peruana Cayetano Heredia

Transcription of TP0126, Treponema pallidum putative OmpW homolog, is regulated by the length of a homopolymeric guanosine repeat

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dc.contributor.author Giacani, L.
dc.contributor.author Brandt, S.L.
dc.contributor.author Ke, W.
dc.contributor.author Reid, T.B.
dc.contributor.author Molini, B.J.
dc.contributor.author Iverson-Cabral, S.
dc.contributor.author Ciccarese, G.
dc.contributor.author Drago, F.
dc.contributor.author Lukehart, S.A.
dc.contributor.author Centurion-Lara, A.
dc.date.accessioned 2019-02-22T14:54:29Z
dc.date.available 2019-02-22T14:54:29Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/5646
dc.description.abstract An effective mechanism for introduction of phenotypic diversity within a bacterial population exploits changes in the length of repetitive DNA elements located within gene promoters. This phenomenon, known as phase variation, causes rapid activation or silencing of gene expression and fosters bacterial adaptation to new or changing environments. Phase variation often occurs in surface-exposed proteins, and in Treponema pallidum subsp. pallidum, the syphilis agent, it was reported to affect transcription of three putative outer membrane protein (OMP)-encoding genes. When the T. pallidum subsp. pallidum Nichols strain genome was initially annotated, the TP0126 open reading frame was predicted to include a poly(G) tract and did not appear to have a predicted signal sequence that might suggest the possibility of its being an OMP. Here we show that the initial annotation was incorrect, that this poly(G) is instead located within the TP0126 promoter, and that it varies in length in vivo during experimental syphilis. Additionally, we show that TP0126 transcription is affected by changes in the poly(G) length consistent with regulation by phase variation. In silico analysis of the TP0126 open reading frame based on the experimentally identified transcriptional start site shortens this hypothetical protein by 69 amino acids, reveals a predicted cleavable signal peptide, and suggests structural homology with the OmpW family of porins. Circular dichroism of recombinant TP0126 supports structural homology to OmpW. Together with the evidence that TP0126 is fully conserved among T. pallidum subspecies and strains, these data suggest an important role for TP0126 in T. pallidum biology and syphilis pathogenesis. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Infection and Immunity
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Animals en_US
dc.subject Rabbits en_US
dc.subject syphilis en_US
dc.subject Syphilis en_US
dc.subject human en_US
dc.subject Recombinant Proteins en_US
dc.subject Article en_US
dc.subject priority journal en_US
dc.subject controlled study en_US
dc.subject genetics en_US
dc.subject metabolism en_US
dc.subject animal experiment en_US
dc.subject animal tissue en_US
dc.subject nonhuman en_US
dc.subject animal en_US
dc.subject gene expression en_US
dc.subject microbiology en_US
dc.subject Gene Expression Regulation, Bacterial en_US
dc.subject Models, Molecular en_US
dc.subject Base Sequence en_US
dc.subject Transcription, Genetic en_US
dc.subject Treponema pallidum en_US
dc.subject Protein Conformation en_US
dc.subject Immunity, Humoral en_US
dc.subject chemistry en_US
dc.subject protein conformation en_US
dc.subject DNA sequence en_US
dc.subject bacterial gene en_US
dc.subject genetic transcription en_US
dc.subject physiology en_US
dc.subject gene expression regulation en_US
dc.subject bacterial strain en_US
dc.subject gene sequence en_US
dc.subject nucleotide sequence en_US
dc.subject open reading frame en_US
dc.subject promoter region en_US
dc.subject recombinant protein en_US
dc.subject chemical structure en_US
dc.subject outer membrane protein en_US
dc.subject Bacterial Outer Membrane Proteins en_US
dc.subject circular dichroism en_US
dc.subject guanosine en_US
dc.subject Guanosine en_US
dc.subject homopolymeric guanosine repeat en_US
dc.subject humoral immunity en_US
dc.subject nucleic acid structure en_US
dc.subject rabbit en_US
dc.subject structural homology en_US
dc.subject TP0126 gene en_US
dc.subject transcription initiation site en_US
dc.subject Transcription Initiation Site en_US
dc.title Transcription of TP0126, Treponema pallidum putative OmpW homolog, is regulated by the length of a homopolymeric guanosine repeat en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/IAI.00360-15
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1098-5522


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