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Development of a lateral flow test for the rapid detection of Avibacterium paragallinarum in chickens suspected of having infectious coryza

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dc.contributor.author Morales Ruiz, Sandra
dc.contributor.author Bendezu, Jorge
dc.contributor.author Choque Guevara, Ricardo
dc.contributor.author Montesinos, Ricardo
dc.contributor.author Requena, David
dc.contributor.author Choque Moreau, Luz
dc.contributor.author Montalvan Avalos, Angela
dc.contributor.author Fernandez-Diaz, Manolo
dc.date.accessioned 2019-03-05T15:23:25Z
dc.date.available 2019-03-05T15:23:25Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/5870
dc.description.abstract Background: Infectious coryza (IC) is an acute respiratory disease of growing chickens and layers caused by Avibacterium paragallinarum. The development of tools that allow rapid pathogen detection is necessary in order to avoid disease dissemination and economic losses in poultry. An Av. paragallinarum-specific Ma-4 epitope of the TonB-dependent transporter (TBDT) was selected using bioinformatic tools in order to immunize a BalbC mouse and to produce monoclonal antibodies to be used in a lateral flow test (LFT) developed for Av. paragallinarum detection in chicken nasal mucus samples. Results: The 1G7G8 monoclonal antibody was able to detect TBDT in Av. paragallinarum cultures (serogroups: A, B and C) by Western blot and indirect ELISA assay. Consequently, we developed a self-pairing prototype LFT. The limit of detection of the prototype LFT using Av. paragallinarum cultures was 1 × 104 colony-forming units (CFU)/mL. Thirty-five nasal mucus samples from chickens suspected of having infectious coryza were evaluated for the LFT detection capacity and compared with bacterial isolation (B.I) and polymerase chain reaction (PCR). Comparative indicators such as sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive values (NPV) and the kappa index (K) were obtained. The values were 100.0% Se, 50% Sp, 65.4% PPV, 100% NPV, and 0.49 K and 83.9% Se, 100% Sp, 100% PPV, 44.4% NPV, and 0.54 K for the comparison of the LFT with B.I and PCR, respectively. Additionally, the LFT allowed the detection of Av. paragallinarum from coinfection cases of Av. paragallinarum with Gallibacterium anatis. Conclusions: The results indicate that the self-pairing prototype LFT is suitable for the detection of TBDT in Av. paragallinarum cultures as well as in field samples such as nasal mucus from Av. paragallinarum-infected chickens. Therefore, this prototype LFT could be considered a rapid and promising tool to be used in farm conditions for Av. paragallinarum diagnosis. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries BMC Veterinary Research
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Sensitivity and Specificity en_US
dc.subject Animals en_US
dc.subject Chickens en_US
dc.subject Poultry Diseases en_US
dc.subject Mice en_US
dc.subject metabolism en_US
dc.subject Bagg albino mouse en_US
dc.subject animal en_US
dc.subject sensitivity and specificity en_US
dc.subject mouse en_US
dc.subject microbiology en_US
dc.subject Mice, Inbred BALB C en_US
dc.subject physiology en_US
dc.subject chicken en_US
dc.subject limit of detection en_US
dc.subject monoclonal antibody en_US
dc.subject Antibodies, Monoclonal en_US
dc.subject bird disease en_US
dc.subject veterinary medicine en_US
dc.subject Avibacterium paragallinarum en_US
dc.subject Haemophilus infection en_US
dc.subject Haemophilus Infections en_US
dc.subject Haemophilus paragallinarum en_US
dc.subject Infectious coryza en_US
dc.subject Lateral flow test (LFT) en_US
dc.subject Limit of Detection en_US
dc.subject Monoclonal antibody en_US
dc.subject TonB-dependent transporter (TBDT) en_US
dc.title Development of a lateral flow test for the rapid detection of Avibacterium paragallinarum in chickens suspected of having infectious coryza en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s12917-018-1729-0
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.00
dc.relation.issn 1746-6148


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