Universidad Peruana Cayetano Heredia
Rapid detection of salmonellosis due to Salmonella enterica serovar Typhimurium in Peruvian commercially bred cavies, using indigenous wild bacteriophages
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| dc.contributor.author | Tamariz, Jesus | |
| dc.contributor.author | Guevara, Victor | |
| dc.contributor.author | Guerra, Humberto | |
| dc.date.accessioned | 2019-03-05T15:23:27Z | |
| dc.date.available | 2019-03-05T15:23:27Z | |
| dc.date.issued | 2018 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/5877 | |
| dc.description.abstract | Introduction: The salmonelloses are among the commonest, most widespread human zoonotic infections. They have generated international networks to attempt their control, since they cause a spectrum of ailments, ranging from inapparent carrier states to full-blown, severe, sometimes deadly diarrheal and systemic disease. Rapid diagnosis is needed for a number of reasons. The aim of this study was to standardize and validate a phage amplification test for the identification of salmonellosis to be applied to infections of Cavia porcellus. Methods: Native bacteriophages were isolated from infected cavies and environmental residues from commercial cavy-breeding facilities. Salmonella enterica serovar Typhimurium ATCC 14028 was used to detect, isolate and propagate the bacteriophages, and to standardize a phage amplification assay to detect S. Typhimurium from rectal swabs of cavies. The phage amplification assay was tested using 2 antiviral agents, MgSO4.7H2O (MAS) and pomegranate rind extract (PRE) plus ferrous sulfate (PRE-FeSO4). Results: The final assay format chosen used PRE-FeSO4 and allowed detection of S. Typhimurium in 90 min from culture, 5 h from clinical samples, with a limit of detection at 10(3) pfu; sensitivity was 98.2%, specificity 98%, negative predictive value (NPV) 96.1%, and positive predictive value (PPV) 99.1%. Conclusion: Bacteriophage amplification is therefore an appropriate, fast procedure for detection of this pathogen in clinical samples. | en_US |
| dc.language.iso | eng | |
| dc.publisher | European Academy of HIV/AIDS and Infectious Diseases | |
| dc.relation.ispartofseries | Germs | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | Salmonella enterica serovar Typhimurium | en_US |
| dc.subject | bacteriophage amplification assay. | en_US |
| dc.subject | bacteriophages | en_US |
| dc.subject | Rapid detection | en_US |
| dc.title | Rapid detection of salmonellosis due to Salmonella enterica serovar Typhimurium in Peruvian commercially bred cavies, using indigenous wild bacteriophages | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.18683/germs.2018.1144 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.03.08 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.02 | |
| dc.relation.issn | 2248-2997 |
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