dc.contributor.author |
Tamariz, Jesus |
|
dc.contributor.author |
Guevara, Victor |
|
dc.contributor.author |
Guerra, Humberto |
|
dc.date.accessioned |
2019-03-05T15:23:27Z |
|
dc.date.available |
2019-03-05T15:23:27Z |
|
dc.date.issued |
2018 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/5877 |
|
dc.description.abstract |
Introduction: The salmonelloses are among the commonest, most widespread human zoonotic infections. They have generated international networks to attempt their control, since they cause a spectrum of ailments, ranging from inapparent carrier states to full-blown, severe, sometimes deadly diarrheal and systemic disease. Rapid diagnosis is needed for a number of reasons. The aim of this study was to standardize and validate a phage amplification test for the identification of salmonellosis to be applied to infections of Cavia porcellus. Methods: Native bacteriophages were isolated from infected cavies and environmental residues from commercial cavy-breeding facilities. Salmonella enterica serovar Typhimurium ATCC 14028 was used to detect, isolate and propagate the bacteriophages, and to standardize a phage amplification assay to detect S. Typhimurium from rectal swabs of cavies. The phage amplification assay was tested using 2 antiviral agents, MgSO4.7H2O (MAS) and pomegranate rind extract (PRE) plus ferrous sulfate (PRE-FeSO4). Results: The final assay format chosen used PRE-FeSO4 and allowed detection of S. Typhimurium in 90 min from culture, 5 h from clinical samples, with a limit of detection at 10(3) pfu; sensitivity was 98.2%, specificity 98%, negative predictive value (NPV) 96.1%, and positive predictive value (PPV) 99.1%. Conclusion: Bacteriophage amplification is therefore an appropriate, fast procedure for detection of this pathogen in clinical samples. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
European Academy of HIV/AIDS and Infectious Diseases |
|
dc.relation.ispartofseries |
Germs |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Salmonella enterica serovar Typhimurium |
en_US |
dc.subject |
bacteriophage amplification assay. |
en_US |
dc.subject |
bacteriophages |
en_US |
dc.subject |
Rapid detection |
en_US |
dc.title |
Rapid detection of salmonellosis due to Salmonella enterica serovar Typhimurium in Peruvian commercially bred cavies, using indigenous wild bacteriophages |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.18683/germs.2018.1144 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.08 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#1.06.02 |
|
dc.relation.issn |
2248-2997 |
|