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Minimally invasive saliva testing to monitor norovirus infection in community settings

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dc.contributor.author Pisanic, N.
dc.contributor.author Ballard, S.-B.
dc.contributor.author Colquechagua, F.D.
dc.contributor.author François, R.
dc.contributor.author Exum, N.
dc.contributor.author Yori, P.P.
dc.contributor.author Schwab, K.J.
dc.contributor.author Granger, D.A.
dc.contributor.author Detrick, B.
dc.contributor.author Olortegui, M.P.
dc.contributor.author Mayta, H.
dc.contributor.author Sánchez, G.J.
dc.contributor.author Gilman, R.H.
dc.contributor.author Heaney, C.D.
dc.contributor.author Vinjé, J.
dc.contributor.author Kosek, M.N.
dc.date.accessioned 2019-03-05T15:23:28Z
dc.date.available 2019-03-05T15:23:28Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/5885
dc.description.abstract Background. Norovirus is a leading cause of acute gastroenteritis worldwide. Routine norovirus diagnosis requires stool collection. Te goal of this study was to develop and validate a noninvasive method to diagnose norovirus to complement stool diagnostics and to facilitate studies on transmission. Methods. A multiplex immunoassay to measure salivary immunoglobulin G (IgG) responses to 5 common norovirus genotypes (GI.1, GII.2, GII.4, GII.6, and GII.17) was developed. Te assay was validated using acute and convalescent saliva samples collected from Peruvian children <5 years of age with polymerase chain reaction (PCR)-diagnosed norovirus infections (n = 175) and controls (n = 32). Te assay sensitivity and specifcity were calculated to determine infection status based on fold rise of salivary norovirus genotype-specifc IgG using norovirus genotype from stool as reference. Results. Te salivary assay detected recent norovirus infections and correctly assigned the infecting genotype. Sensitivity was 71% and specifcity was 96% across the evaluated genotypes compared to PCR-diagnosed norovirus infection. Conclusions. Tis saliva-based assay will be a useful tool to monitor norovirus transmission in high-risk settings such as daycare centers or hospitals. Cross-reactivity is limited between the tested genotypes, which represent the most commonly circulating genotypes. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartof urn:issn:1537-6613
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject antibody detection en_US
dc.subject Article en_US
dc.subject case control study en_US
dc.subject child en_US
dc.subject cohort analysis en_US
dc.subject community care en_US
dc.subject controlled study en_US
dc.subject diagnostic test accuracy study en_US
dc.subject disease activity en_US
dc.subject disease course en_US
dc.subject disease transmission en_US
dc.subject feces analysis en_US
dc.subject female en_US
dc.subject genotype en_US
dc.subject health status en_US
dc.subject human en_US
dc.subject immunoassay en_US
dc.subject major clinical study en_US
dc.subject MAL-ED en_US
dc.subject male en_US
dc.subject minimally invasive procedure en_US
dc.subject Multiplex immunoassay en_US
dc.subject Noninvasive diagnostics en_US
dc.subject Norovirus en_US
dc.subject norovirus infection en_US
dc.subject priority journal en_US
dc.subject receiver operating characteristic en_US
dc.subject reverse transcription polymerase chain reaction en_US
dc.subject Saliva en_US
dc.subject saliva analysis en_US
dc.subject sensitivity and specificity en_US
dc.subject virus identification en_US
dc.title Minimally invasive saliva testing to monitor norovirus infection in community settings en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/infdis/jiy638
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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