dc.contributor.author |
Pisanic, N. |
|
dc.contributor.author |
Ballard, S.-B. |
|
dc.contributor.author |
Colquechagua, F.D. |
|
dc.contributor.author |
François, R. |
|
dc.contributor.author |
Exum, N. |
|
dc.contributor.author |
Yori, P.P. |
|
dc.contributor.author |
Schwab, K.J. |
|
dc.contributor.author |
Granger, D.A. |
|
dc.contributor.author |
Detrick, B. |
|
dc.contributor.author |
Olortegui, M.P. |
|
dc.contributor.author |
Mayta, H. |
|
dc.contributor.author |
Sánchez, G.J. |
|
dc.contributor.author |
Gilman, Robert Hugh |
|
dc.contributor.author |
Heaney, C.D. |
|
dc.contributor.author |
Vinjé, J. |
|
dc.contributor.author |
Kosek, M.N. |
|
dc.date.accessioned |
2019-03-05T15:23:28Z |
|
dc.date.available |
2019-03-05T15:23:28Z |
|
dc.date.issued |
2018 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/5885 |
|
dc.description.abstract |
Background. Norovirus is a leading cause of acute gastroenteritis worldwide. Routine norovirus diagnosis requires stool collection. Te goal of this study was to develop and validate a noninvasive method to diagnose norovirus to complement stool diagnostics and to facilitate studies on transmission. Methods. A multiplex immunoassay to measure salivary immunoglobulin G (IgG) responses to 5 common norovirus genotypes (GI.1, GII.2, GII.4, GII.6, and GII.17) was developed. Te assay was validated using acute and convalescent saliva samples collected from Peruvian children <5 years of age with polymerase chain reaction (PCR)-diagnosed norovirus infections (n = 175) and controls (n = 32). Te assay sensitivity and specifcity were calculated to determine infection status based on fold rise of salivary norovirus genotype-specifc IgG using norovirus genotype from stool as reference. Results. Te salivary assay detected recent norovirus infections and correctly assigned the infecting genotype. Sensitivity was 71% and specifcity was 96% across the evaluated genotypes compared to PCR-diagnosed norovirus infection. Conclusions. Tis saliva-based assay will be a useful tool to monitor norovirus transmission in high-risk settings such as daycare centers or hospitals. Cross-reactivity is limited between the tested genotypes, which represent the most commonly circulating genotypes. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Oxford University Press |
|
dc.relation.ispartofseries |
Journal of Infectious Diseases |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
antibody detection |
en_US |
dc.subject |
Article |
en_US |
dc.subject |
case control study |
en_US |
dc.subject |
child |
en_US |
dc.subject |
cohort analysis |
en_US |
dc.subject |
community care |
en_US |
dc.subject |
controlled study |
en_US |
dc.subject |
diagnostic test accuracy study |
en_US |
dc.subject |
disease activity |
en_US |
dc.subject |
disease course |
en_US |
dc.subject |
disease transmission |
en_US |
dc.subject |
feces analysis |
en_US |
dc.subject |
female |
en_US |
dc.subject |
genotype |
en_US |
dc.subject |
health status |
en_US |
dc.subject |
human |
en_US |
dc.subject |
immunoassay |
en_US |
dc.subject |
major clinical study |
en_US |
dc.subject |
MAL-ED |
en_US |
dc.subject |
male |
en_US |
dc.subject |
minimally invasive procedure |
en_US |
dc.subject |
Multiplex immunoassay |
en_US |
dc.subject |
Noninvasive diagnostics |
en_US |
dc.subject |
Norovirus |
en_US |
dc.subject |
norovirus infection |
en_US |
dc.subject |
priority journal |
en_US |
dc.subject |
receiver operating characteristic |
en_US |
dc.subject |
reverse transcription polymerase chain reaction |
en_US |
dc.subject |
Saliva |
en_US |
dc.subject |
saliva analysis |
en_US |
dc.subject |
sensitivity and specificity |
en_US |
dc.subject |
virus identification |
en_US |
dc.title |
Minimally invasive saliva testing to monitor norovirus infection in community settings |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1093/infdis/jiy638 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.08 |
|
dc.relation.issn |
1537-6613 |
|