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dc.contributor.author | Pisanic, N. | |
dc.contributor.author | Ballard, S.-B. | |
dc.contributor.author | Colquechagua, F.D. | |
dc.contributor.author | François, R. | |
dc.contributor.author | Exum, N. | |
dc.contributor.author | Yori, P.P. | |
dc.contributor.author | Schwab, K.J. | |
dc.contributor.author | Granger, D.A. | |
dc.contributor.author | Detrick, B. | |
dc.contributor.author | Olortegui, M.P. | |
dc.contributor.author | Mayta, H. | |
dc.contributor.author | Sánchez, G.J. | |
dc.contributor.author | Gilman, Robert Hugh | |
dc.contributor.author | Heaney, C.D. | |
dc.contributor.author | Vinjé, J. | |
dc.contributor.author | Kosek, M.N. | |
dc.date.accessioned | 2019-03-05T15:23:28Z | |
dc.date.available | 2019-03-05T15:23:28Z | |
dc.date.issued | 2018 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12866/5885 | |
dc.description.abstract | Background. Norovirus is a leading cause of acute gastroenteritis worldwide. Routine norovirus diagnosis requires stool collection. Te goal of this study was to develop and validate a noninvasive method to diagnose norovirus to complement stool diagnostics and to facilitate studies on transmission. Methods. A multiplex immunoassay to measure salivary immunoglobulin G (IgG) responses to 5 common norovirus genotypes (GI.1, GII.2, GII.4, GII.6, and GII.17) was developed. Te assay was validated using acute and convalescent saliva samples collected from Peruvian children <5 years of age with polymerase chain reaction (PCR)-diagnosed norovirus infections (n = 175) and controls (n = 32). Te assay sensitivity and specifcity were calculated to determine infection status based on fold rise of salivary norovirus genotype-specifc IgG using norovirus genotype from stool as reference. Results. Te salivary assay detected recent norovirus infections and correctly assigned the infecting genotype. Sensitivity was 71% and specifcity was 96% across the evaluated genotypes compared to PCR-diagnosed norovirus infection. Conclusions. Tis saliva-based assay will be a useful tool to monitor norovirus transmission in high-risk settings such as daycare centers or hospitals. Cross-reactivity is limited between the tested genotypes, which represent the most commonly circulating genotypes. | en_US |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.relation.ispartofseries | Journal of Infectious Diseases | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | antibody detection | en_US |
dc.subject | Article | en_US |
dc.subject | case control study | en_US |
dc.subject | child | en_US |
dc.subject | cohort analysis | en_US |
dc.subject | community care | en_US |
dc.subject | controlled study | en_US |
dc.subject | diagnostic test accuracy study | en_US |
dc.subject | disease activity | en_US |
dc.subject | disease course | en_US |
dc.subject | disease transmission | en_US |
dc.subject | feces analysis | en_US |
dc.subject | female | en_US |
dc.subject | genotype | en_US |
dc.subject | health status | en_US |
dc.subject | human | en_US |
dc.subject | immunoassay | en_US |
dc.subject | major clinical study | en_US |
dc.subject | MAL-ED | en_US |
dc.subject | male | en_US |
dc.subject | minimally invasive procedure | en_US |
dc.subject | Multiplex immunoassay | en_US |
dc.subject | Noninvasive diagnostics | en_US |
dc.subject | Norovirus | en_US |
dc.subject | norovirus infection | en_US |
dc.subject | priority journal | en_US |
dc.subject | receiver operating characteristic | en_US |
dc.subject | reverse transcription polymerase chain reaction | en_US |
dc.subject | Saliva | en_US |
dc.subject | saliva analysis | en_US |
dc.subject | sensitivity and specificity | en_US |
dc.subject | virus identification | en_US |
dc.title | Minimally invasive saliva testing to monitor norovirus infection in community settings | en_US |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | https://doi.org/10.1093/infdis/jiy638 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.03.08 | |
dc.relation.issn | 1537-6613 |
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