Modeling the Evans Blue Dilution Method for the Measurement of Plasma Volume in Small Animals: A New Optimized Method

Macarlupu, J.L.; Voituron, N.; Richalet, J.P.; Marchant, D.

dc.contributor.author	Richalet, J.P.
dc.contributor.author	Marchant, D.
dc.contributor.author	Macarlupu, J.L.
dc.contributor.author	Voituron, N.
dc.date.accessioned	2019-04-24T18:23:50Z
dc.date.available	2019-04-24T18:23:50Z
dc.date.issued	2018
dc.identifier.uri	https://hdl.handle.net/20.500.12866/6458
dc.description.abstract	The measurement of plasma volume (V p ) in humans and animals is frequently performed by the Evans blue dye dilution method. However, after injection of Evans blue into the circulation, no steady state is observed because of delayed mixing and progressive leakage of dye out of vascular space. Various methods of calculation have been proposed, either with a single blood sampling 5–10 min after dye injection (Single point method), or with extrapolation at time zero of a logarithmic decay (Log linear method). We propose a method based on a two-compartment hypothesis taking into account the initial mixing and the leakage phase in the time course of dye concentration. Nineteen Sprague–Dawley rats were studied in various conditions and blood sampling was performed before and 2, 4 and 6 min after injection of 200 μg Evans blue. A mathematical model was designed to describe the two-compartment hypothesis and allowed the calculation of V p and K out (rate of disappearance of dye from vascular space). A Bland and Altman representation evidenced an overestimation of V p with previous methods and the great dispersion of results with the single point method, especially when using the 6 min point. Calculation of K out revealed more accurate with the model than the Log linear method, especially when the mixing rate is slow. We suggest using the two-compartment model to measure V p with Evans blue technique in rats. This method also allows precise evaluation of the rate of dye leakage, which could be a good marker of vascular permeability to albumin.	en_US
dc.language.iso	eng
dc.publisher	Springer
dc.relation.ispartofseries	Annals of Biomedical Engineering
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Blood	en_US
dc.subject	Mathematical models	en_US
dc.subject	Rating	en_US
dc.subject	Rats	en_US
dc.subject	Dilution technique	en_US
dc.subject	Dye concentration	en_US
dc.subject	Evans Blue	en_US
dc.subject	Logarithmic decay	en_US
dc.subject	Plasma volume	en_US
dc.subject	Single point methods	en_US
dc.subject	Two-compartment models	en_US
dc.subject	Vascular permeability	en_US
dc.subject	Mixing	en_US
dc.subject	Evans blue	en_US
dc.subject	coloring agent	en_US
dc.subject	Evans blue	en_US
dc.subject	accuracy	en_US
dc.subject	animal experiment	en_US
dc.subject	Article	en_US
dc.subject	calibration	en_US
dc.subject	centrifugation	en_US
dc.subject	chemical analysis	en_US
dc.subject	concentration (parameters)	en_US
dc.subject	dilution	en_US
dc.subject	hypoxia	en_US
dc.subject	nonhuman	en_US
dc.subject	plasma volume	en_US
dc.subject	priority journal	en_US
dc.subject	process optimization	en_US
dc.subject	rat	en_US
dc.subject	animal	en_US
dc.subject	biological model	en_US
dc.subject	Sprague Dawley rat	en_US
dc.subject	Animals	en_US
dc.subject	Coloring Agents	en_US
dc.subject	Evans Blue	en_US
dc.subject	Models, Biological	en_US
dc.subject	Plasma Volume	en_US
dc.subject	Rats, Sprague-Dawley	en_US
dc.title	Modeling the Evans Blue Dilution Method for the Measurement of Plasma Volume in Small Animals: A New Optimized Method	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1007/s10439-018-02114-y
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.04.02
dc.relation.issn	1573-9686