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Modeling the Evans Blue Dilution Method for the Measurement of Plasma Volume in Small Animals: A New Optimized Method

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dc.contributor.author Richalet, J.P.
dc.contributor.author Marchant, D.
dc.contributor.author Macarlupu, J.L.
dc.contributor.author Voituron, N.
dc.date.accessioned 2019-04-24T18:23:50Z
dc.date.available 2019-04-24T18:23:50Z
dc.date.issued 2018
dc.identifier.uri https://hdl.handle.net/20.500.12866/6458
dc.description.abstract The measurement of plasma volume (V p ) in humans and animals is frequently performed by the Evans blue dye dilution method. However, after injection of Evans blue into the circulation, no steady state is observed because of delayed mixing and progressive leakage of dye out of vascular space. Various methods of calculation have been proposed, either with a single blood sampling 5–10 min after dye injection (Single point method), or with extrapolation at time zero of a logarithmic decay (Log linear method). We propose a method based on a two-compartment hypothesis taking into account the initial mixing and the leakage phase in the time course of dye concentration. Nineteen Sprague–Dawley rats were studied in various conditions and blood sampling was performed before and 2, 4 and 6 min after injection of 200 μg Evans blue. A mathematical model was designed to describe the two-compartment hypothesis and allowed the calculation of V p and K out (rate of disappearance of dye from vascular space). A Bland and Altman representation evidenced an overestimation of V p with previous methods and the great dispersion of results with the single point method, especially when using the 6 min point. Calculation of K out revealed more accurate with the model than the Log linear method, especially when the mixing rate is slow. We suggest using the two-compartment model to measure V p with Evans blue technique in rats. This method also allows precise evaluation of the rate of dye leakage, which could be a good marker of vascular permeability to albumin. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:1573-9686
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Blood en_US
dc.subject Mathematical models en_US
dc.subject Rating en_US
dc.subject Rats en_US
dc.subject Dilution technique en_US
dc.subject Dye concentration en_US
dc.subject Evans Blue en_US
dc.subject Logarithmic decay en_US
dc.subject Plasma volume en_US
dc.subject Single point methods en_US
dc.subject Two-compartment models en_US
dc.subject Vascular permeability en_US
dc.subject Mixing en_US
dc.subject Evans blue en_US
dc.subject coloring agent en_US
dc.subject Evans blue en_US
dc.subject accuracy en_US
dc.subject animal experiment en_US
dc.subject Article en_US
dc.subject calibration en_US
dc.subject centrifugation en_US
dc.subject chemical analysis en_US
dc.subject concentration (parameters) en_US
dc.subject dilution en_US
dc.subject hypoxia en_US
dc.subject nonhuman en_US
dc.subject plasma volume en_US
dc.subject priority journal en_US
dc.subject process optimization en_US
dc.subject rat en_US
dc.subject animal en_US
dc.subject biological model en_US
dc.subject Sprague Dawley rat en_US
dc.subject Animals en_US
dc.subject Coloring Agents en_US
dc.subject Evans Blue en_US
dc.subject Models, Biological en_US
dc.subject Plasma Volume en_US
dc.subject Rats, Sprague-Dawley en_US
dc.title Modeling the Evans Blue Dilution Method for the Measurement of Plasma Volume in Small Animals: A New Optimized Method en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s10439-018-02114-y
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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