DSpace Repository

Predominant Mycobacterium tuberculosis families and high rates of recent transmission among new cases are not associated with primary multidrug resistance in Lima, Peru

Show simple item record

dc.contributor.author Barletta, F.
dc.contributor.author Otero Vegas, Larissa
dc.contributor.author de Jong, B.C.
dc.contributor.author Iwamoto, T.
dc.contributor.author Arikawa, K.
dc.contributor.author Van der Stuyft, P.
dc.contributor.author Niemann, S.
dc.contributor.author Merker, M.
dc.contributor.author Uwizeye, C.
dc.contributor.author Seas Ramos, Carlos Rafael
dc.contributor.author Rigouts, L.
dc.date.accessioned 2019-04-24T18:23:53Z
dc.date.available 2019-04-24T18:23:53Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/6471
dc.description.abstract Sputum samples from new tuberculosis (TB) cases were collected over 2 years as part of a prospective study in the northeastern part of Lima, Peru. To measure the contribution of recent transmission to the high rates of multidrug resistance (MDR) in this area, Mycobacterium tuberculosis complex (MTBc) isolates were tested for drug susceptibility to first-line drugs and were genotyped by spoligotyping and 15-locus mycobacterial interspersed repetitive-unit (MIRU-15)-variable-number tandem repeat (VNTR) analysis. MDR was found in 6.8% of 844 isolates, of which 593 (70.3%) were identified as belonging to a known MTBc lineage, whereas 198 isolates (23.5%) could not be assigned to these lineages and 12 (1.4%) represented mixed infections. Lineage 4 accounted for 54.9% (n=463) of the isolates, most of which belonged to the Haarlem family (n=279). MIRU-15 analysis grouped 551/791 isolates (69.7%) in 102 clusters, with sizes ranging from 2 to 46 strains. The overall high clustering rate suggests a high level of recent transmission in this population, especially among younger patients (odds ratio [OR], 1.6; P=0.01). Haarlem strains were more prone to cluster, compared to the other families taken together (OR, 2.0; P<0.0001), while Beijing (OR, 0.6; P=0.006) and LAM (OR, 0.7; P=0.07) strains clustered less. Whereas streptomycin-resistant strains were more commonly found in clusters (OR, 1.8; P=0.03), clustering rates did not differ between MDR and non-MDR strains (OR, 1.8; P=0.1). Furthermore, only 16/51 MDR strains clustered with other MDR strains, suggesting that patients with primary MDR infections acquired the infections mostly from index cases outside the study population, such as retreated cases. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject ethambutol en_US
dc.subject isoniazid en_US
dc.subject rifampicin en_US
dc.subject streptomycin en_US
dc.subject tuberculostatic agent en_US
dc.subject adult en_US
dc.subject aged en_US
dc.subject Article en_US
dc.subject bacterial transmission en_US
dc.subject bacterium examination en_US
dc.subject bacterium identification en_US
dc.subject bacterium isolate en_US
dc.subject China en_US
dc.subject controlled study en_US
dc.subject female en_US
dc.subject gene locus en_US
dc.subject genotype en_US
dc.subject geographic distribution en_US
dc.subject human en_US
dc.subject infection rate en_US
dc.subject major clinical study en_US
dc.subject male en_US
dc.subject multidrug resistance en_US
dc.subject mycobacterial interspersed repetitive unit typing en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject nonhuman en_US
dc.subject Peru en_US
dc.subject population structure en_US
dc.subject priority journal en_US
dc.subject spoligotyping en_US
dc.subject variable number of tandem repeat en_US
dc.subject drug effects en_US
dc.subject genetics en_US
dc.subject microbiology en_US
dc.subject molecular epidemiology en_US
dc.subject multidrug resistance en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject prospective study en_US
dc.subject sputum en_US
dc.subject transmission en_US
dc.subject tuberculosis en_US
dc.subject young adult en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Mycobacterium tuberculosis complex en_US
dc.subject Adult en_US
dc.subject Antitubercular Agents en_US
dc.subject Drug Resistance, Multiple en_US
dc.subject Female en_US
dc.subject Humans en_US
dc.subject Male en_US
dc.subject Molecular Epidemiology en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Peru en_US
dc.subject Prospective Studies en_US
dc.subject Sputum en_US
dc.subject Tuberculosis en_US
dc.subject Young Adult en_US
dc.title Predominant Mycobacterium tuberculosis families and high rates of recent transmission among new cases are not associated with primary multidrug resistance in Lima, Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.03585-14
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1098-660X


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics