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Azithromycin resistance levels and mechanisms in Escherichia coli

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dc.contributor.author Gomes, Claudia
dc.contributor.author Ruiz-Roldan, Lidia
dc.contributor.author Mateu, Judit
dc.contributor.author Ochoa, Theresa J.
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2019-07-04T16:59:21Z
dc.date.available 2019-07-04T16:59:21Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6730
dc.description.abstract Despite azithromycin being used in some countries to treat infections caused by Gram-negative pathogens, no resistance breakpoint for Escherichia coli exists. The aim of this study was to analyse the levels and mechanisms of azithromycin resistance in E. coli. The presence of chromosomal (rplD, rplV and 23S rRNA) mutations, 10 macrolide resistance genes (MRGs) and efflux pump overexpression was determined in 343 E. coli isolates. Overall, 89 (25.9%) isolates had MICs ≥ 32 mg/L to azithromycin, decreasing to 42 (12.2%) when assayed in the presence of Phe-Arg-β-Napthylamide, with 35 of these 42 possessing at least one MRG. Efflux pumps played a role in azithromycin resistance affecting the Minimal Inhibitory Concentration (MIC) levels of 91.2% isolates whereas chromosomal alterations seem to have a minimal role. At least one MRG was found in 22.7% of the isolates with mph(A) being the most commonly found gene. The mph(A) gene plays the main role in the development of azithromycin resistance and 93% of the mph(A)-carrying isolates showed a MIC of 32 mg/L. In the absence of a specific resistance breakpoint our results suggest a MIC of 32 mg/L to be considered in order to detect isolates carrying mechanisms able to confer azithromycin resistance. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:2045-2322
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Antimicrobial resistance en_US
dc.subject Bacterial infection en_US
dc.title Azithromycin resistance levels and mechanisms in Escherichia coli en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1038/s41598-019-42423-3
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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