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Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes

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dc.contributor.author Prajapati, Surendra Kumar
dc.contributor.author Borlon, Celine
dc.contributor.author Rovira-Vallbona, Eduard
dc.contributor.author Gruszczyk, Jakub
dc.contributor.author Menant, Sebastien
dc.contributor.author Tham, Wai-Hong
dc.contributor.author Kattenberg, Johanna Helena
dc.contributor.author Villasis Mayuri, Elizabeth Melisa
dc.contributor.author De Meulenaere, Katlijn
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Vinetz, Joseph Michael
dc.contributor.author Fujita, Ricardo
dc.contributor.author Xuan, Xa Nguyen
dc.contributor.author Urbano Ferreira, Marcelo
dc.contributor.author Nino, Carlos H.
dc.contributor.author Patarroyo, Manuel A.
dc.contributor.author Spanakos, Gregory
dc.contributor.author Kestens, Luc
dc.contributor.author Abbeele, Jan Van Den
dc.contributor.author Rosanas-Urgell, Anna
dc.date.accessioned 2019-07-04T16:59:26Z
dc.date.available 2019-07-04T16:59:26Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6754
dc.description.abstract Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria. en_US
dc.language.iso eng
dc.publisher Springer Nature
dc.relation.ispartofseries Scientific Reports
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Genetic linkage study en_US
dc.subject Malaria en_US
dc.title Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes en_US
dc.type info:eu-repo/semantics/review
dc.identifier.doi https://doi.org/10.1038/s41598-019-45228-6
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 2045-2322


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