Cytokine expression in Treponema pallidum infection

Kojima, N.; Maecker, H.; Klausner, J. D.; Siebert, J. C.; Rosenberg-Hasson, Y.; Konda, K. A.; Vargas Rivera, Silver Keith; Caceres Palacios, Carlos Fernando; León Sandoval, Segundo Ramos

dc.contributor.author	Kojima, N.
dc.contributor.author	Siebert, J. C.
dc.contributor.author	Maecker, H.
dc.contributor.author	Rosenberg-Hasson, Y.
dc.contributor.author	León Sandoval, Segundo Ramos
dc.contributor.author	Vargas Rivera, Silver Keith
dc.contributor.author	Konda, K. A.
dc.contributor.author	Caceres Palacios, Carlos Fernando
dc.contributor.author	Klausner, J. D.
dc.date.accessioned	2019-07-04T16:59:27Z
dc.date.available	2019-07-04T16:59:27Z
dc.date.issued	2019
dc.identifier.uri	https://hdl.handle.net/20.500.12866/6760
dc.description.abstract	Background. Current syphilis tests cannot distinguish between active and past syphilis among patients with serofast rapid plasma reagin (RPR) titers. We investigated whether cytokine profiles might provide insight in the differentiation of active and treated syphilis. Methods. We collected quarterly serum samples from participants at risk for incident syphilis in a prospective cohort study of men and male-to-female transgender women. We defined incident syphilis as a new RPR titer1:8 or a fourfold increase from a prior RPR titer and a positive Treponema pallidum particle agglutination assay. We measured cytokine expression using a 63-multiplex bead-based Luminex assay (eBiosciences/Affymetrix, San Diego, California, USA). We used tertile bins and Chi square tests to identify differences in proportions of cytokines between samples from patients with active and treated syphilis. We constructed a network of cytokine profiles from those findings. We used R software (R version 3.4.1, R, Vienna, Austria) to fit models. Results. We identified 20 pairs of cytokines (out of 1953 possible pairs) that differed between active and treated syphilis. From those, we identified three cytokine networks of interest: an Eotaxin-Rantes-Leptin network, a Mig-IL1ra-Trail-CD40L network, and an IL12p40-IL12p70 network. Conclusions. Differences in cytokine profiles are present among men and male-to-female transgender women with active and treated syphilis. Cytokine assays may be a potentially useful tool for identifying active syphilis among patients with serologic syphilis reactivity.	en_US
dc.language.iso	eng
dc.publisher	BioMed Central
dc.relation.ispartofseries	Journal of Translational Medicine
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	Cytokine	en_US
dc.subject	Cytokines	en_US
dc.subject	Syphilis	en_US
dc.subject	Treponema pallidum	en_US
dc.title	Cytokine expression in Treponema pallidum infection	en_US
dc.type	info:eu-repo/semantics/review
dc.identifier.doi	https://doi.org/10.1186/s12967-019-1947-7
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#1.06.03
dc.relation.issn	1479-5876