Development and validation of a copro-enzyme–linked immunosorbent assay sandwich for detection of echinococcus granulosus–soluble membrane antigens in dogs

Jara Salazar, Luis Miguel; Gavidia, Cesar M.; Rodriguez, Magaly; Gimenez-Lirola, Luis G.; Gilman, Robert Hugh; Verastegui Pimentel, Manuela Renee; Altamirano, Faride; Herrera, Antonio

dc.contributor.author	Jara Salazar, Luis Miguel
dc.contributor.author	Rodriguez, Magaly
dc.contributor.author	Altamirano, Faride
dc.contributor.author	Herrera, Antonio
dc.contributor.author	Verastegui Pimentel, Manuela Renee
dc.contributor.author	Gimenez-Lirola, Luis G.
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Gavidia, Cesar M.
dc.date.accessioned	2019-07-04T16:59:29Z
dc.date.available	2019-07-04T16:59:29Z
dc.date.issued	2019
dc.identifier.uri	https://hdl.handle.net/20.500.12866/6766
dc.description.abstract	Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme–linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus–soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-E. granulosus polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra- and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9–99.6) and 98.2% (CI: 89.5–100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7–100) and 98% (CI: 94.1–100), respectively. No cross-reactivity with Taenia hydatigena, Dipylidium caninum, or Toxocara canis was observed. Intra- and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with E. granulosus. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	adult	en_US
dc.subject	animal experiment	en_US
dc.subject	antigen purification	en_US
dc.subject	Article	en_US
dc.subject	controlled study	en_US
dc.subject	coproantigen sandwich enzyme linked immunosorbent assay	en_US
dc.subject	cross reaction	en_US
dc.subject	Dipylidium caninum	en_US
dc.subject	dog	en_US
dc.subject	echinococcosis	en_US
dc.subject	Echinococcus granulosus	en_US
dc.subject	enzyme linked immunosorbent assay	en_US
dc.subject	feces analysis	en_US
dc.subject	immunoglobulin G antibody	en_US
dc.subject	larval stage	en_US
dc.subject	male	en_US
dc.subject	measurement precision	en_US
dc.subject	membrane antigen	en_US
dc.subject	nonhuman	en_US
dc.subject	parasite identification	en_US
dc.subject	parasite load	en_US
dc.subject	process optimization	en_US
dc.subject	receiver operating characteristic	en_US
dc.subject	sensitivity and specificity	en_US
dc.subject	Taenia hydatigena	en_US
dc.subject	Toxocara canis	en_US
dc.title	Development and validation of a copro-enzyme–linked immunosorbent assay sandwich for detection of echinococcus granulosus–soluble membrane antigens in dogs	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.18-0645
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645