Universidad Peruana Cayetano Heredia

Development and validation of a copro-enzyme–linked immunosorbent assay sandwich for detection of echinococcus granulosus–soluble membrane antigens in dogs

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dc.contributor.author Jara Salazar, Luis Miguel
dc.contributor.author Rodriguez, Magaly
dc.contributor.author Altamirano, Faride
dc.contributor.author Herrera, Antonio
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Gimenez-Lirola, Luis G.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Gavidia, Cesar M.
dc.date.accessioned 2019-07-04T16:59:29Z
dc.date.available 2019-07-04T16:59:29Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6766
dc.description.abstract Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme–linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus–soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-E. granulosus polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra- and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9–99.6) and 98.2% (CI: 89.5–100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7–100) and 98% (CI: 94.1–100), respectively. No cross-reactivity with Taenia hydatigena, Dipylidium caninum, or Toxocara canis was observed. Intra- and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with E. granulosus. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject adult en_US
dc.subject animal experiment en_US
dc.subject antigen purification en_US
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject coproantigen sandwich enzyme linked immunosorbent assay en_US
dc.subject cross reaction en_US
dc.subject Dipylidium caninum en_US
dc.subject dog en_US
dc.subject echinococcosis en_US
dc.subject Echinococcus granulosus en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject feces analysis en_US
dc.subject immunoglobulin G antibody en_US
dc.subject larval stage en_US
dc.subject male en_US
dc.subject measurement precision en_US
dc.subject membrane antigen en_US
dc.subject nonhuman en_US
dc.subject parasite identification en_US
dc.subject parasite load en_US
dc.subject process optimization en_US
dc.subject receiver operating characteristic en_US
dc.subject sensitivity and specificity en_US
dc.subject Taenia hydatigena en_US
dc.subject Toxocara canis en_US
dc.title Development and validation of a copro-enzyme–linked immunosorbent assay sandwich for detection of echinococcus granulosus–soluble membrane antigens in dogs en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.18-0645
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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