Objectives: Dyslipidemia is the major risk factor for cardiovascular disease (CVD), therefore successful control of lipid levels to reduce the risks of CVD is needed. "Kaniwa" (Chenopodium pallidicaule) is a pseudo-cereal cultivated in the Peruvian Andes in Puno. Several studies have shown effect of "kaniwa" on CVD, most of them are attributed to phenolic compounds. On the other hand, there is evidence that there is a decrease in serum cholesterol levels by intake of protein from pseudo-cereals. We isolated kaniwa protein and evaluated the effect on lipid profile of mice with induced hypercholesterolemia. Methods: Kaniwa seeds were ground to obtain flour, it was defatted with hexane. An aqueous solution (1:5 w/v) was brought to pH 9 with 0.1 M NaOH, after centrifugation the supernatant was acidified (pH4.6 with 0.1 M HCl) to precipitate the protein. It was neutralized and then lyophilized. Male mice BALB/c 6 weeks old were distributed in five groups: control diet (CD), control diet and cholesterol (CDC), 2.5% kaniwa protein and cholesterol (2.5% KP), 5% kaniwa protein (5% KP), control diet+cholesterol+atorvastatin (CDCA), furthermore CDC, 2.5% KP, 5% KP and CDCA received by orogastric tube 63.5mg/kg of cholesterol. All kind of diet had 21% of protein but a different source. After 8 weeks all mice were sacrificed and blood was obtained by cardiac puncture, serum cholesterol, HDL-cholesterol and LDL-cholesterol were measured. Results: The isolated was 83.9% of protein. There was a difference in body weight gain among groups during the trial, KP diet group gained less body weight. Serum total cholesterol level and LDL - c of KP diet groups were significantly lower than the CDC group; nevertheless, they were not lower than CDCA group. 5% KP diet was much better to reduce serum cholesterol level. Conclusions: Kaniwa protein diet groups gained less body weight compared to the CDC group. KP diet was able to reduce serum cholesterol and LDL - c levels, however, the effect was not better than atorvastatin group.