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Evaluation of cross-reactivity to Taenia hydatigena and Echinococcus granulosus in the enzyme-linked immunoelectrotransfer blot assay for the diagnosis of porcine cysticercosis

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dc.contributor.author Gomez-Puerta, Lucho
dc.contributor.author Vargas-Calla, Ana
dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Lopez-Urbina, Maria Teresa
dc.contributor.author Dorny, Pierre
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author O'Neal, Seth E.
dc.date.accessioned 2019-07-04T16:59:33Z
dc.date.available 2019-07-04T16:59:33Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6790
dc.description.abstract BACKGROUND: Taenia solium is an important zoonotic parasite that infects humans as definitive host (taeniasis) and pigs as intermediate host (cysticercosis). Serological diagnosis of porcine cysticercosis is limited to antigen detection using ELISA, which is known to cross-react with other Taenia species, and antibody detection using the lentil-lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB), which has not been adequately evaluated for cross-reactivity to other parasites. Field studies suggest that the GP50 diagnostic band of the LLGP EITB may cross-react to Taenia hydatigena, a common non-zoonotic parasitic infection of pigs. The objective of this study was to evaluate the specificity of the LLGP EITB assay in pigs infected experimentally with T. hydatigena and Echinococcus granulosus. RESULTS: Twelve three-month-old seronegative were divided into two groups; six were each given an oral challenge with a single gravid proglottid of T. hydatigena and the other six were each given an oral challenge with 50 gravid proglottids of E. granulosus. Serum samples were collected biweekly until 14 weeks when all pigs underwent a detailed necropsy. Taenia hydatigena cysticerci were found in two of six pigs from the first group. Four T. hydatigena-exposed pigs were seropositive at the GP50-band only on EITB LLGP; two of these had cysts at necropsy while no seronegative pigs had cysts. One E. granulosus-exposed pig was positive to EITB LLGP, again with reactivity only to GP50; all six pigs had hepatic echinococcosis on necropsy. CONCLUSION: These results provide definitive evidence that the GP50 diagnostic band in pigs cross-reacts with T. hydatigena. Evidence of cross-reaction with E. granulosus was not conclusive. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries Parasites and Vectors
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject animal en_US
dc.subject animal experiment en_US
dc.subject animal tissue en_US
dc.subject Animals en_US
dc.subject Antigens, Helminth en_US
dc.subject Article en_US
dc.subject autopsy en_US
dc.subject controlled study en_US
dc.subject cross reaction en_US
dc.subject Cross Reactions en_US
dc.subject Cross-reaction en_US
dc.subject cyst (resting stage) en_US
dc.subject cysticercosis en_US
dc.subject Cysticercosis en_US
dc.subject echinococcosis en_US
dc.subject Echinococcosis en_US
dc.subject Echinococcus granulosus en_US
dc.subject enzyme immunoassay en_US
dc.subject Enzyme-linked immunoelectrotransfer blot en_US
dc.subject epitope en_US
dc.subject Epitopes en_US
dc.subject evaluation study en_US
dc.subject experimental infection en_US
dc.subject glycoprotein en_US
dc.subject GP50 en_US
dc.subject helminth protein en_US
dc.subject Helminth Proteins en_US
dc.subject immunoblotting en_US
dc.subject Immunoblotting en_US
dc.subject immunology en_US
dc.subject lentil lectin glycoprotein enzyme linked immunoelectrotransfer blot assay en_US
dc.subject nonhuman en_US
dc.subject parasite antigen en_US
dc.subject parasitology en_US
dc.subject pig en_US
dc.subject Porcine en_US
dc.subject procedures en_US
dc.subject protein GP50 en_US
dc.subject sensitivity and specificity en_US
dc.subject Specificity en_US
dc.subject Swine en_US
dc.subject swine disease en_US
dc.subject Swine Diseases en_US
dc.subject Taenia en_US
dc.subject Taenia hydatigena en_US
dc.subject Taenia solium en_US
dc.subject taeniasis en_US
dc.subject Taeniasis en_US
dc.subject unclassified drug en_US
dc.subject veterinary medicine en_US
dc.title Evaluation of cross-reactivity to Taenia hydatigena and Echinococcus granulosus in the enzyme-linked immunoelectrotransfer blot assay for the diagnosis of porcine cysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s13071-018-3279-5
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1756-3305

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