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IL-22–expressing CD4+AhR+ T lymphocytes are associated with RANKL-mediated alveolar bone resorption during experimental periodontitis

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dc.contributor.author Monasterio, Gustavo
dc.contributor.author Budini, Victoria
dc.contributor.author Fernandez, Baltasar
dc.contributor.author Castillo, Francisca
dc.contributor.author Rojas, Carolina
dc.contributor.author Alvarez, Carla
dc.contributor.author Cafferata, Emilio A.
dc.contributor.author Vicencio, Emiliano
dc.contributor.author Cortes, Bastian I.
dc.contributor.author Cortez, Cristian
dc.contributor.author Vernal, Rolando
dc.date.accessioned 2019-07-04T17:00:16Z
dc.date.available 2019-07-04T17:00:16Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6826
dc.description.abstract BACKGROUND AND OBJECTIVE: Over the past few years, the importance of interleukin-22 (IL-22) and T-helper (Th)22 lymphocytes in the pathogenesis of periodontitis has become apparent; however, there are still aspects that are not addressed yet. Cells expressing IL-22 and aryl hydrocarbon receptor (AhR), transcription factor master switch gene implicated in the differentiation and function of Th22 lymphocytes, have been detected in periodontal tissues of periodontitis-affected patients. In addition, IL-22 has been associated with osteoclast differentiation and their bone resorptive activity in vitro. However, the destructive potential of IL-22-expressing AhR(+) Th22 lymphocytes over periodontal tissues during periodontitis has not been demonstrated in vivo yet. Therefore, this study aimed to analyze whether IL-22-expressing CD4(+) AhR(+) T lymphocytes detected in periodontal lesions are associated with alveolar bone resorption during experimental periodontitis. MATERIAL AND METHODS: Using a murine model of periodontitis, the expression levels of IL-22 and AhR, as well as the Th1-, Th2-, Th17- and T regulatory-associated cytokines, were analyzed in periodontal lesions using qPCR. The detection of CD4(+) IL-22(+) AhR(+) T lymphocytes was analyzed in periodontal lesions and cervical lymph nodes that drain these periodontal lesions using flow cytometry. In addition, the expression of the osteoclastogenic mediator called receptor activator of nuclear factor-kappaB ligand (RANKL) was analyzed by qPCR, western blot, and immunohistochemistry. Finally, alveolar bone resorption was analyzed using micro-computed tomography and scanning electron microscopy, and the bone resorption levels were correlated with IL-22 and RANKL expression. RESULTS: Higher levels of IL-22, AhR, and RANKL, as well as IL-1beta, IL-6, IL-12, IL-17. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof urn:issn:1600-0765
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject alveolar bone resorption en_US
dc.subject interleukin-22 en_US
dc.subject periodontitis en_US
dc.subject RANKL en_US
dc.subject Th22 lymphocytes en_US
dc.title IL-22–expressing CD4+AhR+ T lymphocytes are associated with RANKL-mediated alveolar bone resorption during experimental periodontitis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/jre.12654
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.14


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