In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed with Dolichos biflorus by flow cytometry.

Castaneda, Sergio; Bravo, Zeze; Cancho, Christian; Valdivia, Martha; Woll, Patricia; Gonzales Rengifo, Gustavo Francisco; Santiani, Alexei; Limaymanta, Orlando; Reyes, Jhakelin

dc.contributor.author	Valdivia, Martha
dc.contributor.author	Reyes, Jhakelin
dc.contributor.author	Bravo, Zeze
dc.contributor.author	Cancho, Christian
dc.contributor.author	Castaneda, Sergio
dc.contributor.author	Limaymanta, Orlando
dc.contributor.author	Woll, Patricia
dc.contributor.author	Santiani, Alexei
dc.contributor.author	Gonzales Rengifo, Gustavo Francisco
dc.date.accessioned	2019-07-04T17:00:16Z
dc.date.available	2019-07-04T17:00:16Z
dc.date.issued	2019
dc.identifier.uri	https://hdl.handle.net/20.500.12866/6831
dc.description.abstract	Spermatogonial stem cell (SSC) is known for its self-renewal capacity. We have studied the in vitro proliferation of isolated SSC from adult alpaca (Vicugna pacos) testes. A total of 107 samples were evaluated of which 31 were evaluated at baseline, 36 were cultivated in DMEM and 40 in STEMPRO media. Half of the cultivated samples was analysed after 14 days, and the rest after 21 days. Round cell subpopulations were identified with FITC-DBA by flow cytometry: strongly positive DBA (sDBA+) as SSC, weakly positive DBA (wDBA+) as in early differentiation and negative DBA as differentiated. At the beginning, 4.16% of the cells were SSC, 37.61% wDBA+ while 54.12% were DBA-. After 14 days, 42.28% of SSC, 44.68% wDBA+ and 11.07% DBA- were found in DMEM while 47.09% of SSC, 32.57% wDBA+ and 18.48% DBA- in STEMPRO. After 21 days 38.66% were SSC, 52.78% wDBA and 7.65% DBA- in DMEM and on STEMPRO media 47.92% SSC, 44.20% wDBA+, 4.93% DBA-. There is a significant difference between the number of initial and SSC cultivated, as well as between DBA- (p < 0.05), while there is no significant difference between the wDBA+ (p > 0.05). Our results suggest that both culture media are appropriate for the in vitro proliferation of alpacas SSC.	en_US
dc.description.sponsorship	Este trabajo fue financiado por la Universidad Nacional Mayor de San Marcos [número de contrato 161001091; B17100101].	es_PE
dc.language.iso	eng
dc.publisher	Wiley
dc.relation.ispartofseries	Andrologia
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	adult	en_US
dc.subject	alpaca	en_US
dc.subject	animal cell	en_US
dc.subject	Article	en_US
dc.subject	cell culture	en_US
dc.subject	cell differentiation	en_US
dc.subject	cell subpopulation	en_US
dc.subject	controlled study	en_US
dc.subject	DBA	en_US
dc.subject	Dolichos	en_US
dc.subject	Dolichos biflorus	en_US
dc.subject	flow cytometry	en_US
dc.subject	fluorescein isothiocyanate	en_US
dc.subject	immunoconglutinin	en_US
dc.subject	in vitro culture	en_US
dc.subject	male	en_US
dc.subject	nonhuman	en_US
dc.subject	spermatogonial stem cell	en_US
dc.subject	spermatogonium	en_US
dc.title	In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed with Dolichos biflorus by flow cytometry.	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1111/and.13269
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.02.01
dc.relation.issn	1439-0272