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In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed with Dolichos biflorus by flow cytometry.

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dc.contributor.author Valdivia, Martha
dc.contributor.author Reyes, Jhakelin
dc.contributor.author Bravo, Zeze
dc.contributor.author Cancho, Christian
dc.contributor.author Castaneda, Sergio
dc.contributor.author Limaymanta, Orlando
dc.contributor.author Woll, Patricia
dc.contributor.author Santiani, Alexei
dc.contributor.author Gonzales, Gustavo F.
dc.date.accessioned 2019-07-04T17:00:16Z
dc.date.available 2019-07-04T17:00:16Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6831
dc.description.abstract Spermatogonial stem cell (SSC) is known for its self-renewal capacity. We have studied the in vitro proliferation of isolated SSC from adult alpaca (Vicugna pacos) testes. A total of 107 samples were evaluated of which 31 were evaluated at baseline, 36 were cultivated in DMEM and 40 in STEMPRO media. Half of the cultivated samples was analysed after 14 days, and the rest after 21 days. Round cell subpopulations were identified with FITC-DBA by flow cytometry: strongly positive DBA (sDBA+) as SSC, weakly positive DBA (wDBA+) as in early differentiation and negative DBA as differentiated. At the beginning, 4.16% of the cells were SSC, 37.61% wDBA+ while 54.12% were DBA-. After 14 days, 42.28% of SSC, 44.68% wDBA+ and 11.07% DBA- were found in DMEM while 47.09% of SSC, 32.57% wDBA+ and 18.48% DBA- in STEMPRO. After 21 days 38.66% were SSC, 52.78% wDBA and 7.65% DBA- in DMEM and on STEMPRO media 47.92% SSC, 44.20% wDBA+, 4.93% DBA-. There is a significant difference between the number of initial and SSC cultivated, as well as between DBA- (p < 0.05), while there is no significant difference between the wDBA+ (p > 0.05). Our results suggest that both culture media are appropriate for the in vitro proliferation of alpacas SSC. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof urn:issn:1439-0272
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject adult en_US
dc.subject alpaca en_US
dc.subject animal cell en_US
dc.subject Article en_US
dc.subject cell culture en_US
dc.subject cell differentiation en_US
dc.subject cell subpopulation en_US
dc.subject controlled study en_US
dc.subject DBA en_US
dc.subject Dolichos en_US
dc.subject Dolichos biflorus en_US
dc.subject flow cytometry en_US
dc.subject fluorescein isothiocyanate en_US
dc.subject immunoconglutinin en_US
dc.subject in vitro culture en_US
dc.subject male en_US
dc.subject nonhuman en_US
dc.subject spermatogonial stem cell en_US
dc.subject spermatogonium en_US
dc.title In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed with Dolichos biflorus by flow cytometry. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/and.13269
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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