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In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata

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dc.contributor.author Palma, Sandra
dc.contributor.author Chile, Nancy
dc.contributor.author Carmen-Orozco, Rogger P.
dc.contributor.author Trompeter, Grace
dc.contributor.author Fishbeck, Kayla
dc.contributor.author Cooper, Virginia
dc.contributor.author Rapoport, Laura
dc.contributor.author Bernal-Teran, Edson G.
dc.contributor.author Condori, Beth J.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.date.accessioned 2019-07-04T17:00:16Z
dc.date.available 2019-07-04T17:00:16Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6832
dc.description.abstract Taenia solium is known to cause human cysticercosis while T. saginata does not. Comparative in vitro and in vivo studies on the oncosphere and the postoncospheral (PO) forms of T. solium and T. saginata may help to elucidate why cysticercosis can occur from one and not the other. The aim of this study was to use in vitro culture assays and in vivo models to study the differences in the development of the T. solium and T. saginata oncosphere. Furthermore, this study aimed to evaluate the expression of cytokines and metalloproteinases (MMPs) in human peripheral blood mononuclear cells (PBMCs), which were stimulated by these oncospheres and PO antigens. T. solium and T. saginata activated oncospheres (AO) were cultured in INT-407 and HCT-8 intestinal cells for 180 days. The T. solium began to die while the T. saginata grew for 180 days and developed to cysticerci in INT-407 cells. Rats were inoculated intracranially with AO and PO forms of either T. saginata or T. solium. Rats infected with T. solium AO and PO forms developed neurocysticercosis (NCC), while those infected with the T. saginata did not. Human PMBCs were stimulated with antigens of AO and PO forms of both species, and the production of cytokines and metalloproteinases (MMPs) was measured. The T. solium AO antigen stimulated a higher production of IL-4, IL-5, en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartof urn:issn:1935-2735
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Animals en_US
dc.subject Disease Models, Animal en_US
dc.subject Rats en_US
dc.subject Cell Line en_US
dc.subject Healthy Volunteers en_US
dc.subject Blood-Brain Barrier/physiology en_US
dc.subject Blood/immunology en_US
dc.subject Cytokines/analysis en_US
dc.subject Epithelial Cells/parasitology en_US
dc.subject Leukocytes, Mononuclear/immunology en_US
dc.subject Metalloproteases/analysis en_US
dc.subject Models, Biological en_US
dc.subject Permeability en_US
dc.subject Taenia saginata/growth & development/pathogenicity en_US
dc.subject Taenia solium/growth & development/pathogenicity en_US
dc.subject Taeniasis/parasitology en_US
dc.title In vitro model of postoncosphere development, and in vivo infection abilities of Taenia solium and Taenia saginata en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0007261
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06

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