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Leishmania species in biopsies of patients with different clinical manifestations identified by high resolution melting and nested PCR in an Endemic district in Peru

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dc.contributor.author Rojas-Jaimes, Jesus
dc.contributor.author Rojas-Palomino, Nyshon
dc.contributor.author Pence, James
dc.contributor.author Lescano, Andres G.
dc.date.accessioned 2019-07-04T17:00:17Z
dc.date.available 2019-07-04T17:00:17Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6843
dc.description.abstract Background: The parasite of the genus Leishmania causes a neglected disease known as leishmaniasis, which has different clinical aspects depending on the species that infects the person and the immune response of the patient. The objective was to determine, using molecular biology, the current circulating species of Leishmania in biopsies of different types of cutaneous leishmaniasis (CL) from the endemic district in the region of “Madre de Dios”, Peru. Methods: The study's 19 samples were obtained in the Huepethue district in the “Madre de Dios” region from patients who were diagnosed for suspected leishmaniasis infection with three clinical manifestations. These were analyzed using kDNA-PCR, Nested-PCR and HRM-PCR to identify the genus of an infecting parasite as well as its species. Results: kDNA-PCR detected all tests for the genus of Leishmania to be positive, while the Nested-PCR could not detect 20.9% of species (one L. (V.) braziliensis and three L. (V.) amazonensis), and the HRM-PCR detected all species presented in the samples. The most prevalent species was L. (V.) braziliensis (73.7%), and the clinical manifestations were ulcers (63.2%), superficial scabs (5.3%) and diffuse form (5.3%), followed by L. (V.) amazonensis (15.8%), manifesting ulcers (15.6%), and L. (V.) lainsoni (10.5%), manifesting superficial scabs (5.3%) and ulcers (5.3%). L. (V.) braziliensis, L. (V.) amazonensis and L. (V.) lainsoni were detected effectively using HRM-PCR in the samples with different clinical manifestations. Conclusions: Highlighting the findings of a high diversity of Leishmania species using High Resolution Melting PCR in biopsies with different clinical manifestations of cutaneous leishmaniasis. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:2405-6731
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Leishmania en_US
dc.subject Peru en_US
dc.subject PCR en_US
dc.subject human en_US
dc.subject adult en_US
dc.subject female en_US
dc.subject male en_US
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject adolescent en_US
dc.subject prevalence en_US
dc.subject nonhuman en_US
dc.subject Leishmania braziliensis en_US
dc.subject Genotyping en_US
dc.subject clinical article en_US
dc.subject polymerase chain reaction en_US
dc.subject human tissue en_US
dc.subject nested polymerase chain reaction en_US
dc.subject skin leishmaniasis en_US
dc.subject clinical feature en_US
dc.subject endemic disease en_US
dc.subject parasite identification en_US
dc.subject skin ulcer en_US
dc.subject stibogluconate sodium en_US
dc.subject Leishmania amazonensis en_US
dc.subject molecular biology en_US
dc.subject skin biopsy en_US
dc.subject skin manifestation en_US
dc.subject Cutaneous leishmaniasis en_US
dc.subject Clinical manifestations en_US
dc.subject high resolution melting analysis en_US
dc.subject kDNA polymerase chain reaction en_US
dc.subject Leishmania lainsoni en_US
dc.title Leishmania species in biopsies of patients with different clinical manifestations identified by high resolution melting and nested PCR in an Endemic district in Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.parepi.2019.e00095
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.09
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08

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