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Three-finger toxins from the venom of Micrurus tschudii tschudii (desert coral snake): Isolation and characterization of tschuditoxin-I.

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dc.contributor.author Lomonte, Bruno
dc.contributor.author Camacho, Erika
dc.contributor.author Fernandez, Julian
dc.contributor.author Salas, Maria
dc.contributor.author Zavaleta Martinez-Vargas, Alfonso
dc.date.accessioned 2019-07-04T17:01:22Z
dc.date.available 2019-07-04T17:01:22Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/6932
dc.description.abstract Venoms from Micrurus (New World coral snakes) display potent peripheral neurotoxicity which may cause death by respiratory paralysis, yet many are poorly or not characterized. The major venom components of coral snakes are three-finger toxins (3FTxs) and phospholipases A2, whose proportions vary among species. As a trend, venoms of Micrurus from South America contain high proportions of 3FTxs, in contrast to most North and Central American species. Micrurus tschudii tschudii, the 'Desert coral snake' from Peru, displays an extreme 3FTx-predominant venom phenotype, with 95% of its proteome belonging to this protein family. This study evaluated the toxicity of its major 3FTxs in mice. A lethal 3FTx, here named tschuditoxin-I, was purified and sequenced by MALDI-TOF-TOF and N-terminal degradation. Tschuditoxin-I showed highest similarity to MS-1, a short-chain alpha-neurotoxin from the aquatic, fish-eating coral snake M. surinamensis. The single amino acid substitution between these two toxins maps at the tip of the first beta-stranded 'finger' in the modeled structure of tschuditoxin-I, suggesting it may have a role in interaction with its target, which remains to be investigated. Owing to its lethal action, tschuditoxin-I is likely to be medically relevant in envenomings. In spite of its 74% sequence identity with an alpha-neurotoxin of M. nigrocinctus, an equine antivenom raised against venom of the latter did not immunorecognize tschuditoxin-I or M. t. tschudii venom by ELISA. This underscores the need of characterizing the biochemical and immunological properties of the main toxic components of Micrurus venoms, aiming to improve the limited para-specific coverage of current antivenoms. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Toxicon
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject adult en_US
dc.subject amino acid substitution en_US
dc.subject amino terminal sequence en_US
dc.subject animal experiment en_US
dc.subject Article en_US
dc.subject biochemical composition en_US
dc.subject controlled study en_US
dc.subject Coral snake en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject immunological parameters en_US
dc.subject isolation en_US
dc.subject matrix-assisted laser desorption-ionization mass spectrometry en_US
dc.subject Micrurus en_US
dc.subject Micrurus t. tschudii en_US
dc.subject Micrurus tschudii tschudii en_US
dc.subject mouse en_US
dc.subject MS 1 neurotoxin en_US
dc.subject nonhuman en_US
dc.subject priority journal en_US
dc.subject protein degradation en_US
dc.subject protein function en_US
dc.subject protein interaction en_US
dc.subject protein purification en_US
dc.subject protein structure en_US
dc.subject sequence analysis en_US
dc.subject snake venom en_US
dc.subject Snake venom en_US
dc.subject South America en_US
dc.subject three finger toxin en_US
dc.subject Three-finger toxins en_US
dc.subject toxin analysis en_US
dc.subject tschuditoxin 1 en_US
dc.subject unclassified drug en_US
dc.title Three-finger toxins from the venom of Micrurus tschudii tschudii (desert coral snake): Isolation and characterization of tschuditoxin-I. en_US
dc.type info:eu-repo/semantics/review
dc.identifier.doi https://doi.org/10.1016/j.toxicon.2019.06.023
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.07
dc.relation.issn 1879-3150


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