Circulating T cell-monocyte complexes are markers of immune perturbations.

Wijewickrama, Ananda; Gilman, Robert Hugh; Saito, Mayuko; Peters, Bjoern; Pomaznoy, Mikhail; Premawansa, Sunil; Vidanagama, Dhammika; deSilva, Aruna D.; Premawansa, Gayani; Taplitz, Randy; Sette, Alessandro; Jung, Yunmin; da Silva Antunes, Ricardo; Tippalagama, Rashmi; Vijayanand, Pandurangan; Weiskopf, Daniela; Gunasena, Bandu; Seumois, Gregory; Greenbaum, Jason A.; Lindestam Arlehamn, Cecilia S.; Burel, Julie G.; Schulten, Veronique; Ley, Klaus; Babor, Mariana

dc.contributor.author	Burel, Julie G.
dc.contributor.author	Pomaznoy, Mikhail
dc.contributor.author	Lindestam Arlehamn, Cecilia S.
dc.contributor.author	Weiskopf, Daniela
dc.contributor.author	da Silva Antunes, Ricardo
dc.contributor.author	Jung, Yunmin
dc.contributor.author	Babor, Mariana
dc.contributor.author	Schulten, Veronique
dc.contributor.author	Seumois, Gregory
dc.contributor.author	Greenbaum, Jason A.
dc.contributor.author	Premawansa, Sunil
dc.contributor.author	Premawansa, Gayani
dc.contributor.author	Wijewickrama, Ananda
dc.contributor.author	Vidanagama, Dhammika
dc.contributor.author	Gunasena, Bandu
dc.contributor.author	Tippalagama, Rashmi
dc.contributor.author	deSilva, Aruna D.
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Saito, Mayuko
dc.contributor.author	Taplitz, Randy
dc.contributor.author	Ley, Klaus
dc.contributor.author	Vijayanand, Pandurangan
dc.contributor.author	Sette, Alessandro
dc.contributor.author	Peters, Bjoern
dc.date.accessioned	2019-12-06T20:57:41Z
dc.date.available	2019-12-06T20:57:41Z
dc.date.issued	2019
dc.identifier.uri	https://hdl.handle.net/20.500.12866/7363
dc.description.abstract	Our results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artifacts and thus ignored in data acquisition and analysis, are the result of biological interaction between immune cells. In particular, we show that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell-monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell:cell complexes should be re-visited.	en_US
dc.language.iso	eng
dc.publisher	eLife Sciences Publications
dc.relation.ispartofseries	eLife
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	human	en_US
dc.subject	flow cytometry	en_US
dc.subject	Article	en_US
dc.subject	controlled study	en_US
dc.subject	gene expression	en_US
dc.subject	gene frequency	en_US
dc.subject	phenotype	en_US
dc.subject	sequence analysis	en_US
dc.subject	confocal microscopy	en_US
dc.subject	artifact	en_US
dc.subject	CD14 antigen	en_US
dc.subject	CD3 antigen	en_US
dc.subject	CD4+ T lymphocyte	en_US
dc.subject	cell population	en_US
dc.subject	cell selection	en_US
dc.subject	cryopreservation	en_US
dc.subject	cytokine response	en_US
dc.subject	dengue	en_US
dc.subject	dengue hemorrhagic fever	en_US
dc.subject	diphtheria pertussis tetanus vaccine	en_US
dc.subject	ex vivo study	en_US
dc.subject	Fc receptor	en_US
dc.subject	immune response	en_US
dc.subject	immunization	en_US
dc.subject	interferon gamma release assay	en_US
dc.subject	latent tuberculosis	en_US
dc.subject	memory T lymphocyte	en_US
dc.subject	monocyte	en_US
dc.subject	nucleotide sequence	en_US
dc.subject	polarization	en_US
dc.subject	polymerase chain reaction	en_US
dc.subject	RNA sequence	en_US
dc.subject	T lymphocyte	en_US
dc.subject	ultrasound	en_US
dc.title	Circulating T cell-monocyte complexes are markers of immune perturbations.	en_US
dc.type	info:eu-repo/semantics/review
dc.identifier.doi	https://doi.org/10.7554/eLife.46045
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#1.06.03
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.01.03
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.01.04
dc.relation.issn	2050-084X