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Susceptibilidad antimicrobiana y mutaciones en el gen ARNr 23s de Helicobacter pylori en pacientes dispépticos

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dc.contributor.author Guzman, Jesus
dc.contributor.author Castillo, Denis
dc.contributor.author Ojeda, Manuel
dc.contributor.author Sauvain, Michel
dc.date.accessioned 2019-12-06T20:57:43Z
dc.date.available 2019-12-06T20:57:43Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7378
dc.description.abstract In order to evaluate antimicrobial susceptibility and detect specific mutations in the 23S rRNA gene in Helicobacter pylori strains, a cross-sectional study was performed on 95 patients with dyspepsia treated in a private clinic in Lima. Antrum biopsies were collected by endoscopy for isolation and evaluation of antimicrobial susceptibility using the broth microdilution method. The detection of specific mutations was developed by PCR-RFLP. The percentage of infection by Helicobacter pylori was 46.3%. Resistance values of 52.3% to clarithromycin, 29.6% to metronidazole, 45.5% to levofloxacin, and 4.6% to amoxicillin were observed. The percentage of specific A2142G and A2143G mutations associated with clarithromycin resistance was 43.5%. In conclusion, we found that antimicrobial resistance rates and the percentage of Helicobacter pylori strains circulating in a private clinic in Lima were high. en_US
dc.description.abstract Con el objetivo de evaluar la susceptibilidad antimicrobiana y detectar mutaciones puntuales en el gen ARNr 23S en cepas de Helicobacter pylori se realizó un estudio transversal que incluyó a 95 pacientes con dispepsia atendidos en una clínica privada de Lima. Mediante endoscopía se colectaron biopsias de antro para el aislamiento de cepas de para la evaluación de la susceptibilidad antimicrobiana empleando la técnica de microdilución en caldo. La detección de mutaciones puntuales se desarrolló mediante PCR-RFLP. El porcentaje de infección por Helicobacter pylori fue de 46,3%, se observaron valores de resistencia de 52,3% a claritromicina, 29,6% a metronidazol, 45,5% a levofloxacino y 4,6% a amoxicilina. El porcentaje de mutaciones puntuales A2142G y A2143G asociados a resistencia a claritromicina fue 43,5%. En conclusión, encontramos que las tasas de resistencia antimicrobiana y el porcentaje de cepas de Helicobacter pylori circulantes en una clínica privada de Lima fueron elevadas. es_PE
dc.language.iso spa
dc.publisher Instituto Nacional de Salud
dc.relation.ispartof urn:issn:1726-4642
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Infecciones por Helicobacter es_PE
dc.subject Claritromicina es_PE
dc.subject Pruebas de sensibilidad microbiana es_PE
dc.subject Farmacorresistencia microbiana es_PE
dc.subject Perú es_PE
dc.title Susceptibilidad antimicrobiana y mutaciones en el gen ARNr 23s de Helicobacter pylori en pacientes dispépticos es_PE
dc.title.alternative Antimicrobial susceptibility and mutations in the 23S rRNA gen of Helicobacter pylori in dyspeptic patients en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.17843/rpmesp.2019.362.3901
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.05


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