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Hindgut microbiota in laboratory-reared and wild Triatoma infestans

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dc.contributor.author Waltmann, Andreea
dc.contributor.author Willcox, Alexandra C.
dc.contributor.author Balasubramanian, Sujata
dc.contributor.author Borrini Mayori, Katty
dc.contributor.author Mendoza Guerrero, Sandra
dc.contributor.author Salazar Sánchez, Renzo Sadath
dc.contributor.author Roach, Jeffrey
dc.contributor.author Condori Pino, Carlos
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Bern, Caryn
dc.contributor.author Juliano, Jonathan J.
dc.contributor.author Levy, Michael Z.
dc.contributor.author Meshnick, Steven R.
dc.contributor.author Bowman, Natalie M.
dc.date.accessioned 2019-12-06T20:57:46Z
dc.date.available 2019-12-06T20:57:46Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7395
dc.description.abstract Triatomine vectors transmit Trypanosoma cruzi, the etiological agent of Chagas disease in humans. Transmission to humans typically occurs when contaminated triatomine feces come in contact with the bite site or mucosal membranes. In the Southern Cone of South America, where the highest burden of disease exists, Triatoma infestans is the principal vector for T. cruzi. Recent studies of other vector-borne illnesses have shown that arthropod microbiota influences the ability of infectious agents to colonize the insect vector and transmit to the human host. This has garnered attention as a potential control strategy against T. cruzi, as vector control is the main tool of Chagas disease prevention. Here we characterized the microbiota in T. infestans feces of both wild-caught and laboratory-reared insects and examined the relationship between microbial composition and T. cruzi infection using highly sensitive high-throughput sequencing technology to sequence the V3-V4 region of the 16S ribosomal RNA gene on the MiSeq Illumina platform. We collected 59 wild (9 with T. cruzi infection) and 10 lab-reared T. infestans (4 with T. cruzi infection) from the endemic area of Arequipa, Perú. Wild T. infestans had greater hindgut bacterial diversity than laboratory-reared bugs. Microbiota of lab insects comprised a subset of those identified in their wild counterparts, with 96 of the total 124 genera also observed in laboratory-reared insects. Among wild insects, variation in bacterial composition was observed, but time and location of collection and development stage did not explain this variation. T. cruzi infection in lab insects did not affect α-or β-diversity; however, we did find that the β-diversity of wild insects differed if they were infected with T. cruzi and identified 10 specific taxa that had significantly different relative abundances in infected vs. uninfected wild T. infestans (Bosea, Mesorhizo-bium, Dietzia, and Cupriavidus were underrepresented in infected bugs; Sporosarcina, an unclassified genus of Porphyromonadaceae, Nestenrenkonia, Alkalibacterium, Peptoniphi-lus, Marinilactibacillus were overrepresented in infected bugs). Our findings suggest that T. cruzi infection is associated with the microbiota of T. infestans and that inferring the microbiota of wild T. infestans may not be possible through sampling of T. infestans reared in the insectary. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Actinobacteria en_US
dc.subject animal en_US
dc.subject Animals en_US
dc.subject arthrodesis en_US
dc.subject arthropod en_US
dc.subject Article en_US
dc.subject Bacteria en_US
dc.subject bacterial DNA en_US
dc.subject bacterium en_US
dc.subject Bacteroidetes en_US
dc.subject Chagas disease en_US
dc.subject Chagas Disease en_US
dc.subject classification en_US
dc.subject cyanobacterium en_US
dc.subject disease transmission en_US
dc.subject DNA extraction en_US
dc.subject DNA, Bacterial en_US
dc.subject feces en_US
dc.subject Feces en_US
dc.subject feces microflora en_US
dc.subject gastrointestinal tract en_US
dc.subject Gastrointestinal Tract en_US
dc.subject gene sequence en_US
dc.subject genetics en_US
dc.subject human en_US
dc.subject Humans en_US
dc.subject insect vector en_US
dc.subject Insect Vectors en_US
dc.subject intestine flora en_US
dc.subject isolation and purification en_US
dc.subject Laboratories en_US
dc.subject laboratory en_US
dc.subject microbial community en_US
dc.subject microbial diversity en_US
dc.subject microbiology en_US
dc.subject Microbiota en_US
dc.subject microflora en_US
dc.subject nonhuman en_US
dc.subject parasitology en_US
dc.subject Peptoniphilus en_US
dc.subject phylogenetic tree en_US
dc.subject phylogeny en_US
dc.subject Phylogeny en_US
dc.subject physiology en_US
dc.subject polymerase chain reaction en_US
dc.subject prevalence en_US
dc.subject Proteobacteria en_US
dc.subject restriction fragment length polymorphism en_US
dc.subject Rhizobiales en_US
dc.subject RNA 16S en_US
dc.subject RNA sequence en_US
dc.subject RNA, Ribosomal, 16S en_US
dc.subject Sanger sequencing en_US
dc.subject time series analysis en_US
dc.subject Triatoma en_US
dc.subject Triatoma infestans en_US
dc.subject Trypanosoma en_US
dc.subject Trypanosoma cruzi en_US
dc.subject vector control en_US
dc.subject Wolbachia en_US
dc.title Hindgut microbiota in laboratory-reared and wild Triatoma infestans en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0007383
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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