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Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes

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dc.contributor.author Valdivia, Martha
dc.contributor.author Castaneda-Zegarra, Sergio
dc.contributor.author Levano, Gloria
dc.contributor.author Lazo, Jorge
dc.contributor.author Reyes, Jhakelin
dc.contributor.author Bravo, Zeze
dc.contributor.author Santiani, Alexei
dc.contributor.author Mujica, Fidel
dc.contributor.author Ruiz, Jaime
dc.contributor.author Gonzales, Gustavo F.
dc.date.accessioned 2019-12-06T20:57:49Z
dc.date.available 2019-12-06T20:57:49Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7435
dc.description.abstract The identification system of spermatogonial stem cell (SSC) was established in alpaca using the molecular expression as well as the reactivity pattern to Dolichos biflorus agglutinin (DBA) by flow cytometry. Twenty-four testicles with their epididymis were recovered from adult alpacas at the slaughterhouse of Huancavelica-Peru. Samples were transported to the Laboratory of Reproductive Physiology at Universidad Nacional Mayor de San Marcos. Testes were selected for our study when the progressive motility of epididymal spermatozoa (ESPM) was above 30%. Isolation of SSC was performed with two enzymatic digestions. Finally, sperm viability was evaluated by means of the trypan blue vital stain in spermatogonial round cells. Samples with more than 80% viability were selected. Isolated cells cultured for 2 days were used for identifying the presence of SSCs by the expression of integrin beta1 (116 bp) and PLZF (206 bp) genes. Spermatogonia were classified according to the DBA reactivity. Spermatogonia with a strong positive to DBA (sDBA(+) ) were classified as SSC (Mean +/- SEM=4.44 +/- 0.68%). Spermatogonia in early differentiation stages stained weakly positive with DBA (wDBA(+) ) (Mean +/- SEM=37.44 +/- 3.07%) and differentiated round cells as DBA negative (Mean +/- SEM=54.12 +/- 3.18%). With the use of molecular and DBA markers, it is possible to identify easily the spermatogonial stem cells in alpaca. en_US
dc.language.iso eng
dc.publisher Wiley
dc.relation.ispartof urn:issn:1439-0272
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject adult en_US
dc.subject agar gel electrophoresis en_US
dc.subject alpaca en_US
dc.subject animal tissue en_US
dc.subject Article en_US
dc.subject beta1 integrin en_US
dc.subject bioinformatics en_US
dc.subject cell differentiation en_US
dc.subject cell membrane en_US
dc.subject controlled study en_US
dc.subject DBA en_US
dc.subject epididymal sperm mobility en_US
dc.subject epididymis en_US
dc.subject flow cytometry en_US
dc.subject male en_US
dc.subject molecular markers en_US
dc.subject n acetylgalactosamine en_US
dc.subject nonhuman en_US
dc.subject sample en_US
dc.subject scanning electron microscopy en_US
dc.subject slaughterhouse en_US
dc.subject spermatogonium en_US
dc.subject spermatozoon en_US
dc.subject SSC en_US
dc.subject trypan blue en_US
dc.subject vital stain en_US
dc.subject zinc finger and BTB domain containing protein 16 en_US
dc.title Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/and.13283
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.01

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