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Molecular characterization of the Taenia solium Tso31 antigen and homologous of other Taenia species from Peru

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dc.contributor.author Vargas-Calla, Ana
dc.contributor.author Gomez-Puerta, Luis A.
dc.contributor.author Lopez, Maria T.
dc.contributor.author Garcia, Hector H.
dc.contributor.author Gonzalez, Armando E.
dc.contributor.author Cysticercosis Working Group in Peru
dc.date.accessioned 2019-12-06T21:02:55Z
dc.date.available 2019-12-06T21:02:55Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7488
dc.description.abstract Several studies have been performed to determine specific antigens for the diagnosis of tapeworms. One of these antigens is Tso31, which is used to differentiate Taenia solium and Taenia saginata in human feces. The aim of the present work was the molecular characterization of this protein in different tapeworm specimens collected in Peru: T. omisa (n = 6), T. hydatigena (n = 7), T. taeniaeformis (n = 4), T. pisiformes (n = 1), T. multiceps (n = 7), and T. solium (n = 10). Total DNA was extracted from each proglottid using a commercial DNA kit for tissue. A nested PCR was used to amplify a fragment of the previously described oncosphere-specific protein Tso31 gene. The nested PCR products were analyzed by 1.5% agarose gel electrophoresis and visualized after ethidium bromide staining. All nested PCR-positive products were sequenced and their sequences were compared. Of all the tapeworms analyzed, only T. solium and T. multiceps amplified the Tso31 gene. All sequences were identical for each species. Our T. solium Tso31 showed 100% similarity when compared with published GenBank sequences. The difference between T. solium and T. multiceps Tso31 samples was 8.1%. In conclusion, our results show that the tsol31 gene is not exclusive to T. solium. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:1432-1955
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject agar gel electrophoresis en_US
dc.subject animal en_US
dc.subject Animals en_US
dc.subject antigen en_US
dc.subject Antigens, Helminth en_US
dc.subject Article en_US
dc.subject Base Sequence en_US
dc.subject DNA en_US
dc.subject DNA sequence en_US
dc.subject DNA, Helminth en_US
dc.subject ethidium bromide en_US
dc.subject feces en_US
dc.subject Feces en_US
dc.subject GenBank en_US
dc.subject genetics en_US
dc.subject helminth DNA en_US
dc.subject Homologous en_US
dc.subject human en_US
dc.subject Humans en_US
dc.subject metabolism en_US
dc.subject molecular biology en_US
dc.subject nested polymerase chain reaction en_US
dc.subject nonhuman en_US
dc.subject nucleotide sequence en_US
dc.subject oncosphere en_US
dc.subject parasite antigen en_US
dc.subject parasitology en_US
dc.subject Peru en_US
dc.subject polymerase chain reaction en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject priority journal en_US
dc.subject Sequence Analysis, DNA en_US
dc.subject staining en_US
dc.subject Taenia en_US
dc.subject Taenia hydatigena en_US
dc.subject Taenia saginata en_US
dc.subject Taenia solium en_US
dc.subject taeniasis en_US
dc.subject Taeniasis en_US
dc.subject Tapeworms en_US
dc.subject Tso31 gene en_US
dc.title Molecular characterization of the Taenia solium Tso31 antigen and homologous of other Taenia species from Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s00436-018-06195-5
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE

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