Performance of a sandwich antigen-detection ELISA for the diagnosis of Porcine Taenia solium cysticercosis

García Lescano, Héctor Hugo; Dorny, P.; Gilman, Robert Hugh; Bustos Palomino, Javier Arturo; Rodriguez, S.; Ninaquispe, B.E.; Yang, S.Y.; Gabriël, S.; Castillo Berrios, Yesenia; Gonzalez Zariquiey, Armando Emiliano

dc.contributor.author	Bustos Palomino, Javier Arturo
dc.contributor.author	Ninaquispe, B.E.
dc.contributor.author	Rodriguez, S.
dc.contributor.author	Castillo Berrios, Yesenia
dc.contributor.author	Yang, S.Y.
dc.contributor.author	Gilman, Robert Hugh
dc.contributor.author	Dorny, P.
dc.contributor.author	Gabriël, S.
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Gonzalez Zariquiey, Armando Emiliano
dc.date.accessioned	2019-12-06T21:02:58Z
dc.date.available	2019-12-06T21:02:58Z
dc.date.issued	2019
dc.identifier.uri	https://hdl.handle.net/20.500.12866/7530
dc.description.abstract	The pig is the natural intermediate host of Taenia solium, a parasite causing significant burden of disease in both humans and pigs. Porcine cysticercosis is traditionally detected via tongue palpation and slaughterhouse meat inspection, both with limited sensitivity. Serum antibody detection has a better performance; however, it does not discriminate past from present infection. Serum antigen detection can demonstrate viable infection and gives a good estimate of parasitic load. This study evaluated a sandwich antigen-detection ELISA using monoclonal antibodies (MoAbs) 158C11 and 60H8 for the diagnosis of viable cysticercosis in pigs. Serum samples were used from 35 naturally T. solium cysticerci–infected pigs, 31 cysticercosis-negative pigs, and 22 pigs with Taenia hydatigena infection (to assess cross-reactions). Positive cysticercosis samples were subcategorized at necropsy according to parasitic burden as mild (1–10 viable cysts, n = 10), moderate (11–100 cysts, n = 5), or severe infection (more than 100 cysts, n = 20). This Ag-ELISA showed a sensitivity of 82.9% and a specificity of 96.8% when not considering cross-reactions with T. hydatigena. Hundred percentage of severely infected, 80% of moderately infected, and 50% of mildly T. solium–infected pigs tested positive. Twenty of 22 pigs with only T. hydatigena infections were positive, with 13 reaching saturating levels in the ELISA. The Ag-ELISA revealed the presence of live cysts and is, thus, a fairly reliable test to monitor experimental infection, response to treatment, and follow-up in animal models of cysticercosis. It should, however, be carefully interpreted when used in regions where T. hydatigena is endemic in pigs.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	animal experiment	en_US
dc.subject	animal model	en_US
dc.subject	Article	en_US
dc.subject	controlled study	en_US
dc.subject	cross reaction	en_US
dc.subject	cysticercosis	en_US
dc.subject	disease severity	en_US
dc.subject	echinococcosis	en_US
dc.subject	enzyme linked immunosorbent assay	en_US
dc.subject	experimental infection	en_US
dc.subject	follow up	en_US
dc.subject	monoclonal antibody	en_US
dc.subject	monoclonal antibody 158C11	en_US
dc.subject	monoclonal antibody 60H8	en_US
dc.subject	nonhuman	en_US
dc.subject	parasite load	en_US
dc.subject	pig	en_US
dc.subject	sensitivity and specificity	en_US
dc.subject	Taenia hydatigena	en_US
dc.subject	Taenia hydatigena infection	en_US
dc.subject	taeniasis	en_US
dc.subject	treatment response	en_US
dc.subject	unclassified drug	en_US
dc.title	Performance of a sandwich antigen-detection ELISA for the diagnosis of Porcine Taenia solium cysticercosis	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.18-0697
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645