Universidad Peruana Cayetano Heredia

Performance of a sandwich antigen-detection ELISA for the diagnosis of Porcine Taenia solium cysticercosis

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dc.contributor.author Bustos Palomino, Javier Arturo
dc.contributor.author Ninaquispe, B.E.
dc.contributor.author Rodriguez, S.
dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Yang, S.Y.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Dorny, P.
dc.contributor.author Gabriël, S.
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.date.accessioned 2019-12-06T21:02:58Z
dc.date.available 2019-12-06T21:02:58Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7530
dc.description.abstract The pig is the natural intermediate host of Taenia solium, a parasite causing significant burden of disease in both humans and pigs. Porcine cysticercosis is traditionally detected via tongue palpation and slaughterhouse meat inspection, both with limited sensitivity. Serum antibody detection has a better performance; however, it does not discriminate past from present infection. Serum antigen detection can demonstrate viable infection and gives a good estimate of parasitic load. This study evaluated a sandwich antigen-detection ELISA using monoclonal antibodies (MoAbs) 158C11 and 60H8 for the diagnosis of viable cysticercosis in pigs. Serum samples were used from 35 naturally T. solium cysticerci–infected pigs, 31 cysticercosis-negative pigs, and 22 pigs with Taenia hydatigena infection (to assess cross-reactions). Positive cysticercosis samples were subcategorized at necropsy according to parasitic burden as mild (1–10 viable cysts, n = 10), moderate (11–100 cysts, n = 5), or severe infection (more than 100 cysts, n = 20). This Ag-ELISA showed a sensitivity of 82.9% and a specificity of 96.8% when not considering cross-reactions with T. hydatigena. Hundred percentage of severely infected, 80% of moderately infected, and 50% of mildly T. solium–infected pigs tested positive. Twenty of 22 pigs with only T. hydatigena infections were positive, with 13 reaching saturating levels in the ELISA. The Ag-ELISA revealed the presence of live cysts and is, thus, a fairly reliable test to monitor experimental infection, response to treatment, and follow-up in animal models of cysticercosis. It should, however, be carefully interpreted when used in regions where T. hydatigena is endemic in pigs. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject animal experiment en_US
dc.subject animal model en_US
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject cross reaction en_US
dc.subject cysticercosis en_US
dc.subject disease severity en_US
dc.subject echinococcosis en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject experimental infection en_US
dc.subject follow up en_US
dc.subject monoclonal antibody en_US
dc.subject monoclonal antibody 158C11 en_US
dc.subject monoclonal antibody 60H8 en_US
dc.subject nonhuman en_US
dc.subject parasite load en_US
dc.subject pig en_US
dc.subject sensitivity and specificity en_US
dc.subject Taenia hydatigena en_US
dc.subject Taenia hydatigena infection en_US
dc.subject taeniasis en_US
dc.subject treatment response en_US
dc.subject unclassified drug en_US
dc.title Performance of a sandwich antigen-detection ELISA for the diagnosis of Porcine Taenia solium cysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.18-0697
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1476-1645


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