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Trypomastigote Excretory Secretory Antigen Blot Is Associated with Trypanosoma cruzi Load and Detects Congenital T. cruzi Infection in Neonates, Using Anti-Shed Acute Phase Antigen Immunoglobulin M

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dc.contributor.author Noazin, Sassan
dc.contributor.author Lee, Jessica A.
dc.contributor.author Malaga, Edith S.
dc.contributor.author Ayala, Edward Valencia
dc.contributor.author Condori, Beth J.
dc.contributor.author Roca, Cristian
dc.contributor.author Lescano, Andres G.
dc.contributor.author Bern, Caryn
dc.contributor.author Castillo, Walter
dc.contributor.author Mayta, Holger
dc.contributor.author Menduiña, Maria Carmen
dc.contributor.author Verastegui, Manuela R.
dc.contributor.author Tinajeros, Freddy
dc.contributor.author Gilman, Robert H.
dc.contributor.author Chagas Working Group in Bolivia and Peru
dc.date.accessioned 2019-12-06T21:02:59Z
dc.date.available 2019-12-06T21:02:59Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7544
dc.description.abstract Background: Congenital Trypanosoma cruzi infection accounts for an estimated 22% of new cases of Chagas disease in Latin America. However, neonatal diagnosis is challenging, as 9-month follow-up for immunoglobulin G testing is poor, quantitative polymerase chain reaction (qPCR) analysis is not routinely performed, and the micromethod misses ≥40% of congenital infections. Methods: Biorepository samples from new mothers and their infants from Piura, Peru, (an area of nonendemicity), and Santa Cruz, Bolivia (an area of endemicity) were accessed. Infant specimens were assessed using the micromethod, qPCR analysis, and a trypomastigote excretory secretory antigen (TESA) blot for detection of immunoglobulin M (IgM)-specific shed acute phase antigen (SAPA) bands, using qPCR as the gold standard. Results: When compared to qPCR, IgM TESA blot was both sensitive and specific for congenital Chagas disease diagnosis. Cumulative sensitivity (whether only 4 bands or all 6 bands were present) was 80% (95% confidence interval [CI], 59%-92%). Specificity was 94% (95% CI, 92%-96%) in the area of endemicity and 100% in the area of nonendemicity. SAPA bands occurred sequentially and in pairs, and parasite loads correlated highly with the number of SAPA bands present. The micromethod detected infection in fewer than half of infected infants. Conclusions: The IgM TESA blot for detection of SAPA bands is rapid, relatively inexpensive, and more sensitive than the micromethod and may be a useful point-of-care test for detection of congenital T. cruzi infection. en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartof urn:issn:1537-6613
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Antibodies, Protozoan en_US
dc.subject blood en_US
dc.subject Bolivia en_US
dc.subject Chagas disease en_US
dc.subject Chagas Disease en_US
dc.subject congenital en_US
dc.subject diagnosis en_US
dc.subject diagnostic test en_US
dc.subject Diagnostic Tests, Routine en_US
dc.subject evaluation study en_US
dc.subject female en_US
dc.subject Female en_US
dc.subject glycoprotein en_US
dc.subject Glycoproteins en_US
dc.subject human en_US
dc.subject Humans en_US
dc.subject IgM SAPA en_US
dc.subject immunoblotting en_US
dc.subject Immunoblotting en_US
dc.subject immunoglobulin M en_US
dc.subject Immunoglobulin M en_US
dc.subject immunology en_US
dc.subject infant en_US
dc.subject Infant en_US
dc.subject Infant, Newborn en_US
dc.subject male en_US
dc.subject Male en_US
dc.subject Neuraminidase en_US
dc.subject newborn en_US
dc.subject Peru en_US
dc.subject pregnancy en_US
dc.subject Pregnancy en_US
dc.subject procedures en_US
dc.subject protozoon antibody en_US
dc.subject sensitivity and specificity en_US
dc.subject Sensitivity and Specificity en_US
dc.subject sialidase en_US
dc.subject TESA blot en_US
dc.subject trans-sialidase en_US
dc.subject Trypanosoma cruzi en_US
dc.title Trypomastigote Excretory Secretory Antigen Blot Is Associated with Trypanosoma cruzi Load and Detects Congenital T. cruzi Infection in Neonates, Using Anti-Shed Acute Phase Antigen Immunoglobulin M en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/infdis/jiy562
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08

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