dc.contributor.author |
Lee, Chan |
|
dc.contributor.author |
Noh, John |
|
dc.contributor.author |
O'Neal, Seth E. |
|
dc.contributor.author |
Gonzalez Zariquiey, Armando Emiliano |
|
dc.contributor.author |
García Lescano, Héctor Hugo |
|
dc.contributor.author |
Handali, Sukwan |
|
dc.date.accessioned |
2019-12-06T21:02:59Z |
|
dc.date.available |
2019-12-06T21:02:59Z |
|
dc.date.issued |
2019 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/7545 |
|
dc.description.abstract |
We developed a novel and portable fluorescent sensor that integrates a lateral flow assay with a quantum dot (Qdots) label and a mobile phone reader for detection of specific antibodies in human serum. We evaluated the utility of this assay to test for antibodies to the Taenia solium rT24H antigen. It was a retrospective study by examining 112 positive human sera from patients with neurocysticercosis (NCC) including samples from patients with single viable cyst (n = 18), two or more viable cysts (n = 71), and subarachnoid (racemose) cysts (n = 23). These samples were collected from previous study subjects in Lima, Peru under an approved study protocol in Peru. The sera were made anonymous under a protocol approved by the CDC Institutional Review Board. Definitive diagnosis of the subject was established by computed-tomography and/or magnetic resonance imaging. To test the specificity of the assay, we evaluated a panel of serum samples obtained from patients with other infections (n = 24), and serum samples from persons in the United States and Egypt who had not traveled outside their country, and therefore are presumed negative for cysticercosis (n = 128). The assay specificity in the negative panel was 99% (95-100%) while assay sensitivity was 89% (79-95%) in NCC patients with two or more viable cysts. Our assay has performance characteristics similar to those of traditional platforms for the detection of NCC and shows promise as a mobile phone reader-based point-of-care test for antibody detection. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Public Library of Science |
|
dc.relation.ispartofseries |
PLoS Neglected Tropical Diseases |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
antibody detection |
en_US |
dc.subject |
antibody response |
en_US |
dc.subject |
Article |
en_US |
dc.subject |
computer assisted tomography |
en_US |
dc.subject |
cross reaction |
en_US |
dc.subject |
culture optimization |
en_US |
dc.subject |
feasibility study |
en_US |
dc.subject |
human |
en_US |
dc.subject |
neurocysticercosis |
en_US |
dc.subject |
nuclear magnetic resonance imaging |
en_US |
dc.subject |
point of care testing |
en_US |
dc.subject |
pyroxylin |
en_US |
dc.subject |
quantum dot |
en_US |
dc.subject |
retrospective study |
en_US |
dc.subject |
sensitivity and specificity |
en_US |
dc.subject |
Taenia solium |
en_US |
dc.title |
Feasibility of a point-of-care test based on quantum dots with a mobile phone reader for detection of antibody responses. |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1371/journal.pntd.0007746 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.06 |
|
dc.relation.issn |
1935-2735 |
|