Universidad Peruana Cayetano Heredia

Feasibility of a point-of-care test based on quantum dots with a mobile phone reader for detection of antibody responses.

Mostrar el registro sencillo del ítem

dc.contributor.author Lee, Chan
dc.contributor.author Noh, John
dc.contributor.author O'Neal, Seth E.
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Handali, Sukwan
dc.date.accessioned 2019-12-06T21:02:59Z
dc.date.available 2019-12-06T21:02:59Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7545
dc.description.abstract We developed a novel and portable fluorescent sensor that integrates a lateral flow assay with a quantum dot (Qdots) label and a mobile phone reader for detection of specific antibodies in human serum. We evaluated the utility of this assay to test for antibodies to the Taenia solium rT24H antigen. It was a retrospective study by examining 112 positive human sera from patients with neurocysticercosis (NCC) including samples from patients with single viable cyst (n = 18), two or more viable cysts (n = 71), and subarachnoid (racemose) cysts (n = 23). These samples were collected from previous study subjects in Lima, Peru under an approved study protocol in Peru. The sera were made anonymous under a protocol approved by the CDC Institutional Review Board. Definitive diagnosis of the subject was established by computed-tomography and/or magnetic resonance imaging. To test the specificity of the assay, we evaluated a panel of serum samples obtained from patients with other infections (n = 24), and serum samples from persons in the United States and Egypt who had not traveled outside their country, and therefore are presumed negative for cysticercosis (n = 128). The assay specificity in the negative panel was 99% (95-100%) while assay sensitivity was 89% (79-95%) in NCC patients with two or more viable cysts. Our assay has performance characteristics similar to those of traditional platforms for the detection of NCC and shows promise as a mobile phone reader-based point-of-care test for antibody detection. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject antibody detection en_US
dc.subject antibody response en_US
dc.subject Article en_US
dc.subject computer assisted tomography en_US
dc.subject cross reaction en_US
dc.subject culture optimization en_US
dc.subject feasibility study en_US
dc.subject human en_US
dc.subject neurocysticercosis en_US
dc.subject nuclear magnetic resonance imaging en_US
dc.subject point of care testing en_US
dc.subject pyroxylin en_US
dc.subject quantum dot en_US
dc.subject retrospective study en_US
dc.subject sensitivity and specificity en_US
dc.subject Taenia solium en_US
dc.title Feasibility of a point-of-care test based on quantum dots with a mobile phone reader for detection of antibody responses. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0007746
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


Ficheros en el ítem

Ficheros Tamaño Formato Ver

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

info:eu-repo/semantics/restrictedAccess Excepto si se señala otra cosa, la licencia del ítem se describe como info:eu-repo/semantics/restrictedAccess

Buscar en el Repositorio


Listar

Panel de Control

Estadísticas