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dc.contributor.author | LaMonte, G.M. | |
dc.contributor.author | Orjuela-Sanchez, P. | |
dc.contributor.author | Calla Choque, Jaeson Santos | |
dc.contributor.author | Wang, L.T. | |
dc.contributor.author | Li, S. | |
dc.contributor.author | Swann, J. | |
dc.contributor.author | Cowell, A.N. | |
dc.contributor.author | Zou, B.Y. | |
dc.contributor.author | Abdel-Haleem Mohamed, A.M. | |
dc.contributor.author | Villa Galarce, Z.H. | |
dc.contributor.author | Moreno, M. | |
dc.contributor.author | Tong Rios, C. | |
dc.contributor.author | Vinetz, Joseph Michael | |
dc.contributor.author | Lewis, N. | |
dc.contributor.author | Winzeler, E.A. | |
dc.date.accessioned | 2019-12-06T21:04:40Z | |
dc.date.available | 2019-12-06T21:04:40Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12866/7558 | |
dc.description.abstract | The exoerythrocytic stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium exoerythrocytic hepatic-stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection. | en_US |
dc.language.iso | eng | |
dc.publisher | Springer Nature | |
dc.relation.ispartofseries | Nature Communications | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
dc.subject | Article | en_US |
dc.subject | biological marker | en_US |
dc.subject | Carcinoma, Hepatocellular | en_US |
dc.subject | cell culture | en_US |
dc.subject | cell line | en_US |
dc.subject | Cell Line | en_US |
dc.subject | Cells, Cultured | en_US |
dc.subject | disease exacerbation | en_US |
dc.subject | genetic transfection | en_US |
dc.subject | genetics | en_US |
dc.subject | heat shock protein 70 | en_US |
dc.subject | hepatocellular carcinoma cell line | en_US |
dc.subject | Hepatocytes | en_US |
dc.subject | host parasite interaction | en_US |
dc.subject | Host-Parasite Interactions | en_US |
dc.subject | HSP70 Heat-Shock Proteins | en_US |
dc.subject | human | en_US |
dc.subject | Humans | en_US |
dc.subject | Immunity, Mucosal | en_US |
dc.subject | immunology | en_US |
dc.subject | liver cell | en_US |
dc.subject | liver cell carcinoma | en_US |
dc.subject | Liver Neoplasms | en_US |
dc.subject | liver tumor | en_US |
dc.subject | malaria | en_US |
dc.subject | Malaria | en_US |
dc.subject | metabolism | en_US |
dc.subject | MUC13 protein, human | en_US |
dc.subject | mucin | en_US |
dc.subject | mucin 13 | en_US |
dc.subject | Mucins | en_US |
dc.subject | mucosal immunity | en_US |
dc.subject | nonhuman | en_US |
dc.subject | parasitology | en_US |
dc.subject | parasitology immunofluorescence assay | en_US |
dc.subject | pathogenicity | en_US |
dc.subject | pathology | en_US |
dc.subject | physiology | en_US |
dc.subject | Plasmodium | en_US |
dc.subject | Plasmodium berghei | en_US |
dc.subject | Plasmodium berghei infection | en_US |
dc.subject | Plasmodium vivax malaria | en_US |
dc.subject | protein expression | en_US |
dc.subject | RNA sequence | en_US |
dc.subject | unclassified drug | en_US |
dc.subject | upregulation | en_US |
dc.title | Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection | en_US |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | https://doi.org/10.1038/s41467-019-08349-0 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.06.03 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.04.00 | |
dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#1.03.00 | |
dc.relation.issn | 2041-1723 |
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