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Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection

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dc.contributor.author LaMonte, G.M.
dc.contributor.author Orjuela-Sanchez, P.
dc.contributor.author Calla Choque, Jaeson Santos
dc.contributor.author Wang, L.T.
dc.contributor.author Li, S.
dc.contributor.author Swann, J.
dc.contributor.author Cowell, A.N.
dc.contributor.author Zou, B.Y.
dc.contributor.author Abdel-Haleem Mohamed, A.M.
dc.contributor.author Villa Galarce, Z.H.
dc.contributor.author Moreno, M.
dc.contributor.author Tong Rios, C.
dc.contributor.author Vinetz, Joseph Michael
dc.contributor.author Lewis, N.
dc.contributor.author Winzeler, E.A.
dc.date.accessioned 2019-12-06T21:04:40Z
dc.date.available 2019-12-06T21:04:40Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7558
dc.description.abstract The exoerythrocytic stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium exoerythrocytic hepatic-stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection. en_US
dc.language.iso eng
dc.publisher Springer Nature
dc.relation.ispartofseries Nature Communications
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Article en_US
dc.subject biological marker en_US
dc.subject Carcinoma, Hepatocellular en_US
dc.subject cell culture en_US
dc.subject cell line en_US
dc.subject Cell Line en_US
dc.subject Cells, Cultured en_US
dc.subject disease exacerbation en_US
dc.subject genetic transfection en_US
dc.subject genetics en_US
dc.subject heat shock protein 70 en_US
dc.subject hepatocellular carcinoma cell line en_US
dc.subject Hepatocytes en_US
dc.subject host parasite interaction en_US
dc.subject Host-Parasite Interactions en_US
dc.subject HSP70 Heat-Shock Proteins en_US
dc.subject human en_US
dc.subject Humans en_US
dc.subject Immunity, Mucosal en_US
dc.subject immunology en_US
dc.subject liver cell en_US
dc.subject liver cell carcinoma en_US
dc.subject Liver Neoplasms en_US
dc.subject liver tumor en_US
dc.subject malaria en_US
dc.subject Malaria en_US
dc.subject metabolism en_US
dc.subject MUC13 protein, human en_US
dc.subject mucin en_US
dc.subject mucin 13 en_US
dc.subject Mucins en_US
dc.subject mucosal immunity en_US
dc.subject nonhuman en_US
dc.subject parasitology en_US
dc.subject parasitology immunofluorescence assay en_US
dc.subject pathogenicity en_US
dc.subject pathology en_US
dc.subject physiology en_US
dc.subject Plasmodium en_US
dc.subject Plasmodium berghei en_US
dc.subject Plasmodium berghei infection en_US
dc.subject Plasmodium vivax malaria en_US
dc.subject protein expression en_US
dc.subject RNA sequence en_US
dc.subject unclassified drug en_US
dc.subject upregulation en_US
dc.title Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1038/s41467-019-08349-0
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.03
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.04.00
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.03.00
dc.relation.issn 2041-1723

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