Universidad Peruana Cayetano Heredia

A pilot evaluation of alternative procedures to simplify LAMP-based malaria diagnosis in field conditions.

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dc.contributor.author Serra-Casas, Elisa
dc.contributor.author Guetens, Pieter
dc.contributor.author Chiheb, Driss
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Rosanas-Urgell, Anna
dc.date.accessioned 2019-12-06T21:04:41Z
dc.date.available 2019-12-06T21:04:41Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7568
dc.description.abstract Highly-sensitive and field-friendly diagnostic tools are needed for accurate detection of low-density malaria infections. Although loop-mediated isothermal amplification (LAMP) fulfills these conditions, operational challenges are still encountered during pilot population screenings in remote settings when employing Loopamp MALARIA Pan/Pf detection kit (Eiken Chemical Co.). This study evaluates different procedures for the simplification of sample preparation and result reading steps of current LAMP protocols. The reference 'Boil & Spin' (B&S) pre-amplification procedure was compared to three alternative methods, along with a colorimetric staining protocol based on malachite green. Results suggested that the B&S supernatant transference step may be omitted without an impact on test performance, even when colorimetry was incorporated to facilitate results visualization. Procedures skipping centrifugation and/or heat-incubation were proved to be compatible with LAMP-based malaria DNA detection, but resulted in a low-to-moderate decrease in sensitivity and ambiguous result interpretation for the most straightforward protocol. Nevertheless, all simplified LAMP methods could still reach lower limits of detection than the currently used tools for malaria mass-screening (i.e. microscopy and rapid tests), indicating that these alternative strategies may deserve further consideration. This evaluation, therefore, demonstrates the feasibility of skipping some of the main procedural bottlenecks of LAMP-malaria protocols, a much-needed achievement to make point-of-care implementation of molecular diagnostics a reality. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Acta Tropica
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Article en_US
dc.subject centrifugation en_US
dc.subject colorimetry en_US
dc.subject controlled study en_US
dc.subject detection method en_US
dc.subject diagnostic test accuracy study en_US
dc.subject DNA en_US
dc.subject DNA determination en_US
dc.subject heat en_US
dc.subject limit of detection en_US
dc.subject loop mediated isothermal amplification en_US
dc.subject Loop-mediated isothermal amplification (LAMP) en_US
dc.subject malachite green en_US
dc.subject malaria en_US
dc.subject Malaria infection en_US
dc.subject mass screening en_US
dc.subject microscopy en_US
dc.subject nonhuman en_US
dc.subject parasite en_US
dc.subject pilot study en_US
dc.subject Plasmodium en_US
dc.subject point of care testing en_US
dc.subject Point-of-care diagnosis en_US
dc.subject protozoal DNA en_US
dc.subject Sample preparation en_US
dc.subject sensitivity and specificity en_US
dc.subject supernatant en_US
dc.title A pilot evaluation of alternative procedures to simplify LAMP-based malaria diagnosis in field conditions. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.actatropica.2019.105125
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.01
dc.relation.issn 1873-6254


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