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MDR/XDR-TB Colour Test for drug susceptibility testing of Mycobacterium tuberculosis, Northwest Ethiopia.

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dc.contributor.author Shibabaw, Agumas
dc.contributor.author Gelaw, Baye
dc.contributor.author Kelley, Holden V.
dc.contributor.author Tesfaye, Ephrem
dc.contributor.author Balada-Llasat, Joan Miquel
dc.contributor.author Evans, Carlton A.
dc.contributor.author Torrelles, Jordi B.
dc.contributor.author Wang, Shu-Hua
dc.contributor.author Tessema, Belay
dc.date.accessioned 2019-12-06T21:04:46Z
dc.date.available 2019-12-06T21:04:46Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7611
dc.description.abstract BACKGROUND: Appropriate-technology tests are needed for Mycobacterium tuberculosis drug-susceptibility testing (DST) in resource-constrained settings. We evaluated the MDR/XDRTB colour plate thin-layer agar test (TB-CX) for M. tuberculosis DST by directly testing sputum at University of Gondar Hospital. METHODS: Sputum samples were each divided into 2 aliquots. One aliquot was mixed with disinfectant and applied directly to the TB-CX quadrant petri-plate containing culture medium with and without isoniazid, rifampicin or ciprofloxacin. Concurrently, the other aliquot was decontaminated with sodium hydroxide, centrifuged and cultured on Lowenstein-Jensen media, then the stored M. tuberculosis isolates were sub-cultured in BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 for reference DST. RESULTS: TB-CX text yielded DST results for 94% (123/131) of positive samples. For paired DST results, median days from sputum processing to DST was 12 for TB-CX versus 35 for LJ-MGIT (P < 0.001). Compared with LJ-MGIT for isoniazid, rifampicin and MDR-TB, TB-CX test had: 59%, 96% and 95% sensitivity; 96%, 94% and 98% specificity; and 85%, 94% and 98% agreement, respectively. All ciprofloxacin DST results were susceptible by both methods. CONCLUSION: The TB-CX was simple and rapid for M. tuberculosis DST. Discordant DST results may have resulted from sub-optimal storage and different isoniazid concentrations used in TB-CX versus the reference standard test. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:1878-3511
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Colour test en_US
dc.subject drug resistant en_US
dc.subject drug susceptibility testing en_US
dc.subject thin layer agar en_US
dc.subject tuberculosis en_US
dc.title MDR/XDR-TB Colour Test for drug susceptibility testing of Mycobacterium tuberculosis, Northwest Ethiopia. en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ijid.2019.10.041

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