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Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

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dc.contributor.author Muehlig, A.
dc.contributor.author Jahn, I.J.
dc.contributor.author Heidler, J.
dc.contributor.author Jahn, M.
dc.contributor.author Weber, K.
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Cialla-May, D.
dc.contributor.author Popp, J.
dc.date.accessioned 2019-12-06T21:04:48Z
dc.date.available 2019-12-06T21:04:48Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7631
dc.description.abstract The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Applied Sciences
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Drug resistant mycobacteria en_US
dc.subject Lab-on-a-Chip (LoC) en_US
dc.subject Personal medicine en_US
dc.subject Quantitative SERS detection of PZA and POA en_US
dc.subject Surface-enhanced Raman spectroscopy (SERS) en_US
dc.title Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/app9122511
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#2.04.00
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.02.01
dc.relation.issn 2076-3417


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