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Biphasic in vitro maturation (CAPA-IVM) specifically improves the developmental capacity of oocytes from small antral follicles

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dc.contributor.author Sanchez, Flor
dc.contributor.author Le, A.H.
dc.contributor.author Ho, V.N.A.
dc.contributor.author Romero Loyola, Sergio Jean Markos
dc.contributor.author Van Ranst, H.
dc.contributor.author De Vos, M.
dc.contributor.author Gilchrist, R.B.
dc.contributor.author Ho, T.M.
dc.contributor.author Vuong, L.N.
dc.contributor.author Smitz, J.
dc.date.accessioned 2019-12-06T21:04:52Z
dc.date.available 2019-12-06T21:04:52Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/20.500.12866/7654
dc.description.abstract Purpose: To investigate the effectiveness of a biphasic IVM culture strategy at improving IVM outcomes in oocytes from small follicles (< 6 mm) compared with routine Standard IVM in patients with polycystic ovaries. Methods: This prospective pilot study was performed in 40 women with polycystic ovaries whose oocytes were randomized to two IVM culture methods. Patients received a total stimulation dose of 450 IU rFSH. Cumulus-oocyte complexes (COCs) from follicles < 6 mm and ≥ 6 mm were retrieved and cultured separately in either a prematuration medium with c-type natriuretic peptide followed by IVM (CAPA-IVM), or STD-IVM. Primary outcomes were maturation rate, embryo quality, and the number of vitrified day 3 embryos per patient. Results: Use of the CAPA-IVM system led to a significant improvement in oocyte maturation (p < 0.05), to a doubling in percentage of good and top-quality day 3 embryos per COC, and to an increased number of vitrified day 3 embryos (p < 0.001), compared to STD IVM. Oocytes from follicles < 6 mm benefited most from CAPA-IVM, showing a significant increase in the amount of good and top-quality embryos compared to STD IVM. CAPA-IVM yielded significantly (p < 0.0001) less GV-arrested oocytes and larger oocyte diameters (p < 0.05) than STD IVM. Conclusions: CAPA-IVM brings significant improvements in maturation and embryological outcomes, most notably to oocytes from small antral follicles (< 6 mm), which can be easily retrieved from patients with a minimal ovarian stimulation. The study demonstrates the robustness and transferability of the CAPA-IVM method across laboratories and populations. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Journal of Assisted Reproduction and Genetics
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject human en_US
dc.subject embryo en_US
dc.subject IVM en_US
dc.subject priority journal en_US
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject female en_US
dc.subject human cell en_US
dc.subject adult en_US
dc.subject clinical outcome en_US
dc.subject prospective study en_US
dc.subject clinical article en_US
dc.subject antral follicle en_US
dc.subject C-type natriuretic peptide en_US
dc.subject cell count en_US
dc.subject cell size en_US
dc.subject Embryo en_US
dc.subject in vitro oocyte maturation en_US
dc.subject IVF en_US
dc.subject natriuretic peptide type C en_US
dc.subject oocyte development en_US
dc.subject Oocyte prematuration en_US
dc.subject ovary polycystic disease en_US
dc.subject recombinant follitropin en_US
dc.subject vitrification en_US
dc.title Biphasic in vitro maturation (CAPA-IVM) specifically improves the developmental capacity of oocytes from small antral follicles en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s10815-019-01551-5
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.02
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.02
dc.relation.issn 1573-7330


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