Universidad Peruana Cayetano Heredia
Biphasic in vitro maturation (CAPA-IVM) specifically improves the developmental capacity of oocytes from small antral follicles
JavaScript is disabled for your browser. Some features of this site may not work without it.
Mostrar el registro sencillo del ítem
| dc.contributor.author | Sanchez, Flor | |
| dc.contributor.author | Le, A.H. | |
| dc.contributor.author | Ho, V.N.A. | |
| dc.contributor.author | Romero Loyola, Sergio Jean Markos | |
| dc.contributor.author | Van Ranst, H. | |
| dc.contributor.author | De Vos, M. | |
| dc.contributor.author | Gilchrist, R.B. | |
| dc.contributor.author | Ho, T.M. | |
| dc.contributor.author | Vuong, L.N. | |
| dc.contributor.author | Smitz, J. | |
| dc.date.accessioned | 2019-12-06T21:04:52Z | |
| dc.date.available | 2019-12-06T21:04:52Z | |
| dc.date.issued | 2019 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12866/7654 | |
| dc.description.abstract | Purpose: To investigate the effectiveness of a biphasic IVM culture strategy at improving IVM outcomes in oocytes from small follicles (< 6 mm) compared with routine Standard IVM in patients with polycystic ovaries. Methods: This prospective pilot study was performed in 40 women with polycystic ovaries whose oocytes were randomized to two IVM culture methods. Patients received a total stimulation dose of 450 IU rFSH. Cumulus-oocyte complexes (COCs) from follicles < 6 mm and ≥ 6 mm were retrieved and cultured separately in either a prematuration medium with c-type natriuretic peptide followed by IVM (CAPA-IVM), or STD-IVM. Primary outcomes were maturation rate, embryo quality, and the number of vitrified day 3 embryos per patient. Results: Use of the CAPA-IVM system led to a significant improvement in oocyte maturation (p < 0.05), to a doubling in percentage of good and top-quality day 3 embryos per COC, and to an increased number of vitrified day 3 embryos (p < 0.001), compared to STD IVM. Oocytes from follicles < 6 mm benefited most from CAPA-IVM, showing a significant increase in the amount of good and top-quality embryos compared to STD IVM. CAPA-IVM yielded significantly (p < 0.0001) less GV-arrested oocytes and larger oocyte diameters (p < 0.05) than STD IVM. Conclusions: CAPA-IVM brings significant improvements in maturation and embryological outcomes, most notably to oocytes from small antral follicles (< 6 mm), which can be easily retrieved from patients with a minimal ovarian stimulation. The study demonstrates the robustness and transferability of the CAPA-IVM method across laboratories and populations. | en_US |
| dc.language.iso | eng | |
| dc.publisher | Springer | |
| dc.relation.ispartofseries | Journal of Assisted Reproduction and Genetics | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.subject | human | en_US |
| dc.subject | embryo | en_US |
| dc.subject | IVM | en_US |
| dc.subject | priority journal | en_US |
| dc.subject | Article | en_US |
| dc.subject | controlled study | en_US |
| dc.subject | female | en_US |
| dc.subject | human cell | en_US |
| dc.subject | adult | en_US |
| dc.subject | clinical outcome | en_US |
| dc.subject | prospective study | en_US |
| dc.subject | clinical article | en_US |
| dc.subject | antral follicle | en_US |
| dc.subject | C-type natriuretic peptide | en_US |
| dc.subject | cell count | en_US |
| dc.subject | cell size | en_US |
| dc.subject | Embryo | en_US |
| dc.subject | in vitro oocyte maturation | en_US |
| dc.subject | IVF | en_US |
| dc.subject | natriuretic peptide type C | en_US |
| dc.subject | oocyte development | en_US |
| dc.subject | Oocyte prematuration | en_US |
| dc.subject | ovary polycystic disease | en_US |
| dc.subject | recombinant follitropin | en_US |
| dc.subject | vitrification | en_US |
| dc.title | Biphasic in vitro maturation (CAPA-IVM) specifically improves the developmental capacity of oocytes from small antral follicles | en_US |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | https://doi.org/10.1007/s10815-019-01551-5 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.01.02 | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#3.02.02 | |
| dc.relation.issn | 1573-7330 |
Ficheros en el ítem
| Ficheros | Tamaño | Formato | Ver |
|---|---|---|---|
|
No hay ficheros asociados a este ítem. |
|||
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
Artículos en revistas indizadas [4988]
Recuperados de bases de datos bibliográficas
Excepto si se señala otra cosa, la licencia del ítem se describe como info:eu-repo/semantics/restrictedAccess
