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Brucella melitensis T cell epitope recognition in humans with brucellosis in Peru

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dc.contributor.author Cannella, Anthony P.
dc.contributor.author Arlehamn, Cecilia S.Lindestam
dc.contributor.author Sidney, John
dc.contributor.author Patra, Kallash P.
dc.contributor.author Torres Fajardo, Katherine Jessica
dc.contributor.author Tsolis, Renee M.
dc.contributor.author Liang, Li
dc.contributor.author Felgner, Philip L.
dc.contributor.author Saito, Mayuko
dc.contributor.author Gotuzzo Herencia, José Eduardo
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Sette, Alessandro
dc.contributor.author Vinetz, Joseph Michael
dc.date.accessioned 2020-06-10T18:11:30Z
dc.date.available 2020-06-10T18:11:30Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/7956
dc.description.abstract Brucella melitensis, one of the causative agents of human brucellosis, causes acute, chronic, and relapsing infection. While T cell immunity in brucellosis has been extensively studied in mice, no recognized human T cell epitopes that might provide new approaches to classifying and prognosticating B. melitensis infection have ever been delineated. Twenty-seven pools of 500 major histocompatibility complex class II (MHC-II) restricted peptides were created by computational prediction of promiscuous MHC-II CD4+ T cell derived from the top 50 proteins recognized by IgG in human sera on a genome level B. melitensis protein microarray. Gamma interferon (IFN-γ) and interleukin-5 (IL-5) enzyme-linked immunospot (ELISPOT) analyses were used to quantify and compare Th1 and Th2 responses of leukapheresis-obtained peripheral blood mononuclear cells from Peruvian subjects cured after acute infection (n=9) and from patients who relapsed (n=5). Four peptide epitopes derived from 3 B. melitensis proteins (BMEI 1330, a DegP/HtrA protease; BMEII 0029, type IV secretion system component VirB5; and BMEII 0691, a predicted periplasmic binding protein of a peptide transport system) were found repeatedly to produce significant IFN-γ ELISPOT responses in both acute-infection and relapsing patients; none of the peptides distinguished the patient groups. IL-5 responses against the panel of peptides were insignificant. These experiments are the first to systematically identify B. melitensis MHC-II-restricted CD4+ T cell epitopes recognized by the human immune response, with the potential for new approaches to brucellosis diagnostics and understanding the immunopathogenesis related to this intracellular pathogen. © 2014, American Society for Microbiology. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Infection and Immunity
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject acute disease en_US
dc.subject adult en_US
dc.subject article en_US
dc.subject bacterial protein en_US
dc.subject Brucella melitensis en_US
dc.subject brucellosis en_US
dc.subject CD4+ T lymphocyte en_US
dc.subject cellular immunity en_US
dc.subject clinical article en_US
dc.subject cytokine production en_US
dc.subject enzyme linked immunospot assay en_US
dc.subject epitope en_US
dc.subject female en_US
dc.subject gamma interferon en_US
dc.subject human en_US
dc.subject immune response en_US
dc.subject immunoglobulin G en_US
dc.subject immunopathogenesis en_US
dc.subject interleukin 5 en_US
dc.subject leukapheresis en_US
dc.subject major histocompatibility antigen class 2 en_US
dc.subject major histocompatibility complex restriction en_US
dc.subject male en_US
dc.subject nonhuman en_US
dc.subject peripheral blood mononuclear cell en_US
dc.subject Peru en_US
dc.subject priority journal en_US
dc.subject relapse en_US
dc.subject T lymphocyte en_US
dc.subject Th1 cell en_US
dc.subject Th2 cell en_US
dc.title Brucella melitensis T cell epitope recognition in humans with brucellosis in Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/IAI.00796-13
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1098-5522


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