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Field evaluation of the InBios Chagas detect plus rapid test in serum and whole-blood specimens in Bolivia

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dc.contributor.author Shah, Vishal
dc.contributor.author Ferrufino, Lisbeth
dc.contributor.author Gilman, Robert H.
dc.contributor.author Ramirez, Margot
dc.contributor.author Saenza, Eliana
dc.contributor.author Malaga, Edith
dc.contributor.author Sanchez, Gerardo
dc.contributor.author Okamoto, Emi E.
dc.contributor.author Sherbuck, Jacqueline E.
dc.contributor.author Clark, Eva H.
dc.contributor.author Galdos-Cardenas, Gerson
dc.contributor.author Bozo, Ricardo
dc.contributor.author Flores-Franco, Jorge Luis
dc.contributor.author Colanzi, Rony
dc.contributor.author Verastegui, Manuela
dc.contributor.author Bern, Caryn
dc.date.accessioned 2020-06-10T18:11:30Z
dc.date.available 2020-06-10T18:11:30Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/7960
dc.description.abstract Trypanosoma cruzi causes Chagas disease, which affects an estimated 7 million to 8 million people. Chagas disease is endemic throughout Latin America, with the highest prevalence in Bolivia. Conventional diagnosis requires a well-equipped laboratory with experienced personnel. We evaluated the Chagas Detect Plus (CDP) (InBios, Seattle, WA), a rapid immunochromatographic assay for IgG antibodies to T. cruzi. CDP performance was compared to infection status based on results obtained by indirect hemagglutination assay, immunofluorescent-antibody test, and enzyme-linked immunosorbent assay. Confirmed infection required positive results by at least 2 conventional assays. We used specimens from adults of both sexes in a general hospital in the city of Santa Cruz and from pregnant women in a hospital and children in villages in the Bolivian Chaco, an area of hyperendemicity. CDP was performed in paired whole-blood and serum specimens from 385 individuals in the two hospital studies and in 200 serum specimens from the community study. CDP showed sensitivities/specificities of 96.2% (95% confidence interval, 92.7 to 98.4)/98.8% (95.9 to 99.9) in whole blood and 99.3% (97.5 to 99.9)/96.9% (94.2 to 98.6) in serum, with no differences by sex, age group, or study site. CDP showed excellent sensitivity and specificity in our study population, comparable to those of conventional serology. The test is reliable for field surveys, requires no laboratory equipment, and performed well in serum and whole blood. The CDP could also be used for accurate maternal screening to identify neonates at risk of congenital transmission. CDP performance data in diverse geographic areas are needed to strengthen the evidence base for its use. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartof urn:issn:1556-679X
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adolescent en_US
dc.subject Adult en_US
dc.subject Aged en_US
dc.subject Aged, 80 and over en_US
dc.subject Antibodies, Protozoan/blood en_US
dc.subject Bolivia en_US
dc.subject Chagas Disease/diagnosis en_US
dc.subject Child en_US
dc.subject Child, Preschool en_US
dc.subject Chromatography, Affinity/methods en_US
dc.subject Female en_US
dc.subject Humans en_US
dc.subject Infant en_US
dc.subject Male en_US
dc.subject Middle Aged en_US
dc.subject Pregnancy en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Serum en_US
dc.subject Trypanosoma cruzi/immunology en_US
dc.title Field evaluation of the InBios Chagas detect plus rapid test in serum and whole-blood specimens in Bolivia en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/CVI.00609-14
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.03

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