Universidad Peruana Cayetano Heredia

Validation of microscopic observation drug susceptibility testing for rapid, direct rifampicin and isoniazid drug susceptibility testing in patients receiving tuberculosis treatment

Mostrar el registro sencillo del ítem

dc.contributor.author Coronel, J.
dc.contributor.author Roper, M. H.
dc.contributor.author Herrera, C.
dc.contributor.author Bonilla, C.
dc.contributor.author Jave, O.
dc.contributor.author Gianella, C.
dc.contributor.author Sabogal, I.
dc.contributor.author Huancaré, V.
dc.contributor.author Leo, E.
dc.contributor.author Tyas, A.
dc.contributor.author Mendoza-Ticona, A.
dc.contributor.author Caviedes, L.
dc.contributor.author Moore, David Alexander James
dc.date.accessioned 2020-06-10T18:11:31Z
dc.date.available 2020-06-10T18:11:31Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/7967
dc.description.abstract Drug susceptibility testing (DST) is often needed in patients clinically failing tuberculosis (TB) therapy. Most studies of phenotypic direct drug susceptibility tests, such as microscopic observation drug susceptibility (MODS) tests, have been performed in patients not receiving TB treatment. The effect of ongoing TB treatment on the performance of MODS direct DST has not been previously explored, but patients failing such therapy constitute an important target group. The aim of this study was to determine the performance of MODS direct rifampicin and isoniazid DST in patients clinically failing first-line TB treatment, and to compare MODS direct DST with indirect proportion method DST. Sputa from 264 TB patients were cultured in parallel in Lowenstein-Jensen (LJ) and MODS assays; strains were tested for rifampicin and isoniazid susceptibility by the proportion method at the national reference laboratory. Ninety-three samples were culture-positive by LJ and MODS (concordance of 96%; kappa 0.92). With conventional MODS plate DST reading (performed on the same day as the sample is classified as culture-positive), the isoniazid DST concordance was 96.8% (kappa 0.89), and the concordance for rifampicin susceptibility testing was 92.6% (kappa 0.80). Reading of MODS DST plates 1 week after cultures had been determined to be culture-positive improved overall performance marginally-the isoniazid DST concordance was 95.7% (kappa 0.85); and the rifampicin DST concordance was 96.8% (kappa 0.91). Sensitivity for detection of multidrug-resistant TB was 95.8%. MODS testing provided reliable rifampicin and isoniazid DST results for samples obtained from patients receiving TB therapy. A modified DST reading schedule for such samples, with a final reading 1 week after a MODS culture turns positive, marginally improves the concordance with reference DST. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Clinical Microbiology and Infection
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adolescent en_US
dc.subject Adult en_US
dc.subject Female en_US
dc.subject Humans en_US
dc.subject Male en_US
dc.subject Young Adult en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Aged en_US
dc.subject Middle Aged en_US
dc.subject tuberculosis en_US
dc.subject treatment failure en_US
dc.subject Drug Resistance, Bacterial en_US
dc.subject Mycobacterium tuberculosis/drug effects en_US
dc.subject Sputum/microbiology en_US
dc.subject MODS en_US
dc.subject Tuberculosis, Multidrug-Resistant/diagnosis/drug therapy/microbiology en_US
dc.subject Tuberculosis, Pulmonary/diagnosis/drug therapy/microbiology en_US
dc.subject Drug susceptibility test en_US
dc.subject Isoniazid/pharmacology/therapeutic use en_US
dc.subject Microbial Sensitivity Tests/methods en_US
dc.subject Rifampin/pharmacology/therapeutic use en_US
dc.title Validation of microscopic observation drug susceptibility testing for rapid, direct rifampicin and isoniazid drug susceptibility testing in patients receiving tuberculosis treatment en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1111/1469-0691.12401
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1469-0691


Ficheros en el ítem

Ficheros Tamaño Formato Ver

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

info:eu-repo/semantics/restrictedAccess Excepto si se señala otra cosa, la licencia del ítem se describe como info:eu-repo/semantics/restrictedAccess

Buscar en el Repositorio


Listar

Panel de Control

Estadísticas