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Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples

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dc.contributor.author Crannell, Zachary Austin
dc.contributor.author Cabada, Miguel Mauricio
dc.contributor.author Castellanos-Gonzalez, Alejandro
dc.contributor.author Irani, Ayesha
dc.contributor.author White, Arthur Clinton
dc.contributor.author Richards-Kortum, Rebecca
dc.date.accessioned 2020-06-10T18:11:36Z
dc.date.available 2020-06-10T18:11:36Z
dc.date.issued 2015
dc.identifier.uri https://hdl.handle.net/20.500.12866/8017
dc.description.abstract Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. The RPAG assay was then tested in the highlands of Peru on 104 stool samples collected from the surrounding communities where it showed 73% sensitivity and 95% specificity against a polymerase chain reaction and microscopy composite gold standard. Further improvements in clinical sensitivity will be needed for the RPAG assay to have clinical relevance. en_US
dc.language.iso eng
dc.publisher American Society of Tropical Medicine and Hygiene
dc.relation.ispartof urn:issn:1476-1645
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Feces/parasitology en_US
dc.subject Giardia lamblia/isolation & purification en_US
dc.subject Giardiasis/diagnosis/epidemiology en_US
dc.subject Humans en_US
dc.subject Peru/epidemiology en_US
dc.subject Polymerase Chain Reaction/methods en_US
dc.subject Recombinases/chemistry/metabolism en_US
dc.subject Sensitivity and Specificity en_US
dc.title Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.4269/ajtmh.14-0593
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06


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