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Genotyping of potentially pathogenic Acanthamoeba strains isolated from nasal swabs of healthy individuals in Peru

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dc.contributor.author Cabello-Vilchez, Alfonso Martin
dc.contributor.author Martin-Navarro, Carmen Maria
dc.contributor.author Lopez-Arencibia, Atteneri
dc.contributor.author Reyes-Batlle, Maria
dc.contributor.author Gonzalez, Ana C.
dc.contributor.author Guerra, Humberto
dc.contributor.author Gotuzzo, Eduardo
dc.contributor.author Valladares, Basilio
dc.contributor.author Pinero, Jose E.
dc.contributor.author Lorenzo-Morales, Jacob
dc.date.accessioned 2020-06-10T18:11:37Z
dc.date.available 2020-06-10T18:11:37Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/8038
dc.description.abstract Free Living Amoebae (FLA) of Acanthamoeba genus are widely distributed in the environment and can be found in the air, soil and water; and have also been isolated from air-conditioning units. In humans, they are causative agents of a sight-threating infection of the cornea, Acanthamoeba keratitis (AK) and a fatal infection of the central nervous system known as Granulomatous Amoebic Encephalitis (GAE). In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in nasal swabs from individuals in two regions of Peru. Identification of isolates was based on cyst morphology and PCR-sequencing of the Diagnostic Fragment 3 to identify strains at the genotype level. The pathogenic potential of the isolates was also assayed using temperature and osmotolerance assays and extracellular proteases zymograms. The obtained results revealed that all isolated strains exhibited pathogenic potential. After sequencing the highly variable DF3 (Diagnostic Fragment 3) region in the 18S rRNA gene as previously described, genotype T4 was found to be the most common one in the samples included in this study but also genotype T15 was identified. To the best of our knowledge, this is the first study on the characterization of Acanthamoeba strains at the genotype level and the first report of genotype T4 and T15 in Peru. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:1873-6254
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Peru en_US
dc.subject Humans en_US
dc.subject Genotype en_US
dc.subject Acanthamoeba en_US
dc.subject Acanthamoeba/classification/genetics/pathogenicity en_US
dc.subject Genotyping en_US
dc.subject Nasal Mucosa/parasitology en_US
dc.subject Nasal swabs en_US
dc.title Genotyping of potentially pathogenic Acanthamoeba strains isolated from nasal swabs of healthy individuals in Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.actatropica.2013.10.006
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.01 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.01


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