Genotyping of potentially pathogenic Acanthamoeba strains isolated from nasal swabs of healthy individuals in Peru

Guerra, Humberto; Gotuzzo Herencia, José Eduardo; Martín-Navarro, Carmen María; Cabello Vílchez, Alfonso Martín; Valladares, Basilio; López-Arencibia, Atteneri; Lorenzo-Morales, Jacob; Reyes-Batlle, María; González, Ana C.; Piñero, José E.

dc.contributor.author	Cabello Vílchez, Alfonso Martín
dc.contributor.author	Martín-Navarro, Carmen María
dc.contributor.author	López-Arencibia, Atteneri
dc.contributor.author	Reyes-Batlle, María
dc.contributor.author	González, Ana C.
dc.contributor.author	Guerra, Humberto
dc.contributor.author	Gotuzzo Herencia, José Eduardo
dc.contributor.author	Valladares, Basilio
dc.contributor.author	Piñero, José E.
dc.contributor.author	Lorenzo-Morales, Jacob
dc.date.accessioned	2020-06-10T18:11:37Z
dc.date.available	2020-06-10T18:11:37Z
dc.date.issued	2013
dc.identifier.uri	https://hdl.handle.net/20.500.12866/8038
dc.description.abstract	Free Living Amoebae (FLA) of Acanthamoeba genus are widely distributed in the environment and can be found in the air, soil and water; and have also been isolated from air-conditioning units. In humans, they are causative agents of a sight-threating infection of the cornea, Acanthamoeba keratitis (AK) and a fatal infection of the central nervous system known as Granulomatous Amoebic Encephalitis (GAE). In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in nasal swabs from individuals in two regions of Peru. Identification of isolates was based on cyst morphology and PCR-sequencing of the Diagnostic Fragment 3 to identify strains at the genotype level. The pathogenic potential of the isolates was also assayed using temperature and osmotolerance assays and extracellular proteases zymograms. The obtained results revealed that all isolated strains exhibited pathogenic potential. After sequencing the highly variable DF3 (Diagnostic Fragment 3) region in the 18S rRNA gene as previously described, genotype T4 was found to be the most common one in the samples included in this study but also genotype T15 was identified. To the best of our knowledge, this is the first study on the characterization of Acanthamoeba strains at the genotype level and the first report of genotype T4 and T15 in Peru.	en_US
dc.language.iso	eng
dc.publisher	Elsevier
dc.relation.ispartofseries	Acta Tropica
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Peru	en_US
dc.subject	Humans	en_US
dc.subject	Genotype	en_US
dc.subject	Acanthamoeba	en_US
dc.subject	Acanthamoeba/classification/genetics/pathogenicity	en_US
dc.subject	Genotyping	en_US
dc.subject	Nasal Mucosa/parasitology	en_US
dc.subject	Nasal swabs	en_US
dc.title	Genotyping of potentially pathogenic Acanthamoeba strains isolated from nasal swabs of healthy individuals in Peru	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1016/j.actatropica.2013.10.006
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.08
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#4.03.01
dc.relation.issn	1873-6254