Infection of laboratory-colonized Anopheles darlingi mosquitoes by Plasmodium vivax

Vinetz, Joseph Michael; Rodriguez, Hugo; Winzeler, Elizabeth A.; Chuquiyauri, Raul; Moreno, Marta; Tong, Carlos; Meister, Stephan; Llanos Cuentas, Elmer Alejandro; Conn, Jan E.; Gamboa Vilela, Dionicia Baziliza; Maguina, Paula; Guzman Guzman, Mitchel Anthony

dc.contributor.author	Moreno, Marta
dc.contributor.author	Tong, Carlos
dc.contributor.author	Guzman Guzman, Mitchel Anthony
dc.contributor.author	Chuquiyauri, Raul
dc.contributor.author	Llanos Cuentas, Elmer Alejandro
dc.contributor.author	Rodriguez, Hugo
dc.contributor.author	Gamboa Vilela, Dionicia Baziliza
dc.contributor.author	Meister, Stephan
dc.contributor.author	Winzeler, Elizabeth A.
dc.contributor.author	Maguina, Paula
dc.contributor.author	Conn, Jan E.
dc.contributor.author	Vinetz, Joseph Michael
dc.date.accessioned	2020-06-10T18:11:37Z
dc.date.available	2020-06-10T18:11:37Z
dc.date.issued	2014
dc.identifier.uri	https://hdl.handle.net/20.500.12866/8041
dc.description.abstract	Anopheles darlingi Root is the most important malaria vector in the Amazonia region of South America. However, continuous propagation of An. darlingi in the laboratory has been elusive, limiting entomological, genetic/genomic, and vector-pathogen interaction studies of this mosquito species. Here, we report the establishment of an An. darlingi colony derived from wild-caught mosquitoes obtained in the northeastern Peruvian Amazon region of Iquitos in the Loreto Department. We show that the numbers of eggs, larvae, pupae, and adults continue to rise at least to the F6 generation. Comparison of feeding Plasmodium vivax ex vivo of F4 and F5 to F1 generation mosquitoes showed the comparable presence of oocysts and sporozoites, with numbers that corresponded to blood-stage asexual parasitemia and gametocytemia, confirming P. vivax vectorial capacity in the colonized mosquitoes. These results provide new avenues for research on An. darlingi biology and study of An. darlingi-Plasmodium interactions.	en_US
dc.language.iso	eng
dc.publisher	American Society of Tropical Medicine and Hygiene
dc.relation.ispartofseries	American Journal of Tropical Medicine and Hygiene (ASTMH Journal)
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	Plasmodium vivax	en_US
dc.subject	Animals	en_US
dc.subject	Anopheles/parasitology	en_US
dc.subject	Female	en_US
dc.subject	Insect Vectors/parasitology	en_US
dc.subject	Laboratory Animal Science/methods	en_US
dc.subject	Malaria, Vivax/transmission	en_US
dc.subject	Male	en_US
dc.subject	Oocysts	en_US
dc.subject	Sexual Behavior, Animal	en_US
dc.subject	Sporozoites	en_US
dc.title	Infection of laboratory-colonized Anopheles darlingi mosquitoes by Plasmodium vivax	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.4269/ajtmh.13-0708
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1476-1645