Universidad Peruana Cayetano Heredia

Endosymbiotic Mycobacterium chelonae in a Vermamoeba vermiformis strain isolated from the nasal mucosa of an HIV patient in Lima, Peru

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dc.contributor.author Cabello Vílchez, Alfonso Martín
dc.contributor.author Mena, Rosmery
dc.contributor.author Zuñiga, Johanna
dc.contributor.author Cermeño, Pablo
dc.contributor.author Martín-Navarro, Carmen Ma
dc.contributor.author González, Ana C.
dc.contributor.author López-Arencibia, Atteneri
dc.contributor.author Reyes-Batlle, María
dc.contributor.author Piñero, José E.
dc.contributor.author Valladares, Basilio
dc.contributor.author Lorenzo-Morales, Jacob
dc.date.accessioned 2020-06-10T18:11:38Z
dc.date.available 2020-06-10T18:11:38Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8047
dc.description.abstract In March 2010, a 35 year-old HIV/AIDS female patient was admitted to hospital to start treatment with Highly Active Antiretroviral Therapy (HAART) since during a routine control a dramatic decrease in the CD4(+) levels was detected. At this stage, a nasal swab from each nostril was collected from the patient to include it in the samples for the case study mentioned above. Moreover, it is important to mention that the patient was diagnosed in 2009 with invasive pneumococcal disease, acute cholecystitis, pancreatitis and pulmonary tuberculosis. The collected nasal swabs from both nostrils were positive for Vermamoeba vermiformis species which was identified using morphological and PCR/DNA sequencing approaches. Basic Local Alignment Search Tool (BLAST) homology and phylogenetic analysis confirmed the amoebic strain to belong to V.vermiformis species. Molecular identification of the Mycobacterium strain was carried out using a bacterial universal primer pair for the 16S rDNA gene at the genus level and the rpoB gene was amplified and sequenced as previously described to identify the Mycobacterium species (Shin et al., 2008; Sheen et al., 2013). Homology and phylogenetic analyses of the rpoB gene confirmed the species as Mycobacterium chelonae. In parallel, collected swabs were tested by PCR and were positive for the presence of V.vermiformis and M.chelonae. This work describes the identification of an emerging bacterial pathogen,M.chelonae from a Free-Living Amoebae (FLA) strain belonging to the species V.vermiformis that colonized the nasal cavities of an HIV/AIDS patient, previously diagnosed with TB. Awareness within clinicians and public health professionals should be raised, as pathogenic agents such as M.chelonae may be using FLA to propagate and survive in the environment. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Experimental Parasitology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Symbiosis en_US
dc.subject Adult en_US
dc.subject Amebiasis/complications en_US
dc.subject Amoebae en_US
dc.subject Disease Reservoirs en_US
dc.subject DNA, Bacterial/isolation & purification en_US
dc.subject DNA, Protozoan/isolation & purification en_US
dc.subject Female en_US
dc.subject Hartmannella/genetics/isolation & purification/microbiology en_US
dc.subject HIV Infections/complications/microbiology/parasitology en_US
dc.subject Humans en_US
dc.subject Molecular Sequence Data en_US
dc.subject Mycobacterium chelonae en_US
dc.subject Mycobacterium chelonae/genetics/isolation & purification/physiology en_US
dc.subject Mycobacterium Infections, Nontuberculous/complications/transmission en_US
dc.subject Nasal Mucosa/microbiology/parasitology en_US
dc.subject PCR en_US
dc.subject Peru en_US
dc.subject Vermamoeba vermiformis en_US
dc.title Endosymbiotic Mycobacterium chelonae in a Vermamoeba vermiformis strain isolated from the nasal mucosa of an HIV patient in Lima, Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.exppara.2014.02.014
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn 1090-2449


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