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Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain

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dc.contributor.author Reyes-Batlle, M.
dc.contributor.author Todd, C.D.
dc.contributor.author Martín-Navarro, C.M.
dc.contributor.author López-Arencibia, A.
dc.contributor.author Cabello-Vilchez, A.M.
dc.contributor.author González, A.C.
dc.contributor.author Córdoba-Lanús, E.
dc.contributor.author Lindo, J.F.
dc.contributor.author Valladares, B.
dc.contributor.author Piñero, J.E.
dc.contributor.author Lorenzo-Morales, J.
dc.date.accessioned 2020-06-10T18:12:13Z
dc.date.available 2020-06-10T18:12:13Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8052
dc.description.abstract Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains in soil samples collected between 2012 and 2013 in Gran Canaria Island, Canary Islands, Spain. Samples were inoculated onto non-nutrient agar (NNA) plates and were checked for the presence of Acanthamoeba. Identification of Acanthamoeba strains was based on the morphology of the cyst and trophozoite forms. Subsequently, positive samples were cloned for their molecular characterization at the genotype level by sequencing the DF3 region located in the 18S rDNA gene of Acanthamoeba as previously described. Sequencing results revealed the presence of T2, T5 and T4 genotypes within the studied samples. To the best of our knowledge, this is the first report demonstrating the presence of Acanthamoeba in Gran Canaria Island and the first study at the genotype level in the Canary Islands. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:1432-1955
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Genotype en_US
dc.subject morphology en_US
dc.subject priority journal en_US
dc.subject controlled study en_US
dc.subject genotype en_US
dc.subject article en_US
dc.subject nonhuman en_US
dc.subject Spain en_US
dc.subject Acanthamoeba en_US
dc.subject DNA, Protozoan en_US
dc.subject parasite identification en_US
dc.subject nucleotide sequence en_US
dc.subject sequence analysis en_US
dc.subject parasite isolation en_US
dc.subject RNA, Ribosomal, 18S en_US
dc.subject molecular cloning en_US
dc.subject Soil en_US
dc.subject trophozoite en_US
dc.subject Acanthamoeba castellanii en_US
dc.subject DNA, Ribosomal en_US
dc.subject DNA 18S en_US
dc.subject Acanthamoeba palestinensis en_US
dc.subject agar en_US
dc.subject soil inoculation en_US
dc.subject type strain en_US
dc.title Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s00436-014-3778-z
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07

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