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The isolation of Balamuthia mandrillaris from environmental sources from Peru

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dc.contributor.author Cabello-Vílchez, A.M.
dc.contributor.author Reyes-Batlle, M.
dc.contributor.author Montalbán-Sandoval, E.
dc.contributor.author Martín-Navarro, C.Ma.
dc.contributor.author López-Arencibia, A.
dc.contributor.author Elias-Letts, R.
dc.contributor.author Guerra, H.
dc.contributor.author Gotuzzo, E.
dc.contributor.author Martínez-Carretero, E.
dc.contributor.author Piñero, J.E.
dc.contributor.author Maciver, S.K.
dc.contributor.author Valladares, B.
dc.contributor.author Lorenzo-Morales, J.
dc.date.accessioned 2020-06-10T18:12:13Z
dc.date.available 2020-06-10T18:12:13Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8053
dc.description.abstract Balamuthia mandrillaris is an opportunistic free-living amoeba that has been reported to cause skin lesions and the fatal Balamuthia amoebic encephalitis (BAE) in humans and other animals. Currently, around 200 human BAE cases have been reported worldwide, although this number is considered to be underestimated. The highest number of BAE cases has been reported in the American continent, mainly in the southwest of the USA. Peru seems to be another hotspot for BAE with around 55 human cases having been identified, usually involving cutaneous infection, especially lesions in the central face area. The isolation of Balamuthia from environmental sources has been reported on only three prior occasions, twice from Californian soils and once from dust in Iran and so it seems that this amoeba is relatively rarely encountered in samples from the environment. We investigated that possibility of finding the amoebae in soil samples from different regions where clinical cases have been reported in Peru. Twenty-one samples were cultured in non-nutrient agar plates and were checked for the presence of B. mandrillaris-like trophozoites and/or cysts. Those samples that were positive for these amoebae by microscopic criteria were then confirmed by PCR amplification and DNA sequencing of the mitochondrial 16S rDNA gene of B. mandrillaris. We have detected the presence of B. mandrillaris in four samples collected in the regions of Piura (3) and Lima (1) where infection cases have been previously reported. We hypothesize that B. mandrillaris is present in Peru in soil and dust which therefore constitutes a source of the infection for the BAE cases previously reported in this country. Further studies should be carried out in the area to confirm the generality of this finding. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:1432-1955
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Amebiasis en_US
dc.subject amoeba (life cycle stage) en_US
dc.subject Animals en_US
dc.subject article en_US
dc.subject Balamuthia infection en_US
dc.subject Balamuthia mandrillaris en_US
dc.subject controlled study en_US
dc.subject Diagnosis en_US
dc.subject DNA 16S en_US
dc.subject DNA sequence en_US
dc.subject Emerging pathogen en_US
dc.subject Encephalitis en_US
dc.subject gene amplification en_US
dc.subject human en_US
dc.subject Humans en_US
dc.subject major clinical study en_US
dc.subject nonhuman en_US
dc.subject nucleotide sequence en_US
dc.subject parasite isolation en_US
dc.subject Peru en_US
dc.subject priority journal en_US
dc.subject RNA, Protozoan en_US
dc.subject RNA, Ribosomal, 16S en_US
dc.subject Sequence Analysis, DNA en_US
dc.subject Skin en_US
dc.subject skin defect en_US
dc.subject skin infection en_US
dc.subject soil en_US
dc.subject Soil en_US
dc.subject Therapy en_US
dc.subject trophozoite en_US
dc.title The isolation of Balamuthia mandrillaris from environmental sources from Peru en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s00436-014-3900-2
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07

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