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Isolation and molecular characterization of Acanthamoeba and Balamuthia mandrillaris from combination shower units in Costa Rica

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dc.contributor.author Retana-Moreira, L.
dc.contributor.author Abrahams-Sandí, E.
dc.contributor.author Cabello-Vílchez, A.M.
dc.contributor.author Reyes-Batlle, M.
dc.contributor.author Valladares, B.
dc.contributor.author Martínez-Carretero, E.
dc.contributor.author Piñero, J.E.
dc.contributor.author Lorenzo-Morales, J.
dc.date.accessioned 2020-06-10T18:12:13Z
dc.date.available 2020-06-10T18:12:13Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8054
dc.description.abstract Free living amoebae (FLA) are ubiquitous protozoa, which may behave as parasites under certain conditions. Four genera are recognized as causal agents of infections in humans and animals: Naegleria, Sappinia, Acanthamoeba and Balamuthia. This work determines the presence of FLA in combination shower units and employs molecular biology for the characterization of isolates. The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of Acanthamoeba genotype T4 in 30 % of the units sampled. In addition to Acanthamoeba cysts, trophozoites with morphological characteristics similar to Balamuthia were identified. PCR assay using the mitochondrial 16S rRNA gene as a target confirmed the identification of the amoeba as Balamuthia mandrillaris. Up to date, this is the first report of the isolation of B. mandrillaris in Central America and the fifth report worldwide. en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof urn:issn:1432-1955
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Water Supply en_US
dc.subject human en_US
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject genotype en_US
dc.subject genetics en_US
dc.subject nonhuman en_US
dc.subject isolation and purification en_US
dc.subject parasitology en_US
dc.subject Balamuthia mandrillaris en_US
dc.subject Acanthamoeba en_US
dc.subject polymerase chain reaction en_US
dc.subject DNA, Protozoan en_US
dc.subject protozoal DNA en_US
dc.subject gene targeting en_US
dc.subject RNA 16S en_US
dc.subject morphological trait en_US
dc.subject RNA, Ribosomal, 16S en_US
dc.subject water supply en_US
dc.subject Animalia en_US
dc.subject parasite isolation en_US
dc.subject cyst (resting stage) en_US
dc.subject molecular biology en_US
dc.subject Costa Rica en_US
dc.subject amoeba (life cycle stage) en_US
dc.subject Balamuthia en_US
dc.subject trophozoite en_US
dc.subject DNA 18S en_US
dc.subject free living amoebae en_US
dc.subject Free-living amoebae en_US
dc.subject fresh water en_US
dc.subject Fresh Water en_US
dc.subject Naegleria en_US
dc.subject Protozoa en_US
dc.subject Trophozoites en_US
dc.title Isolation and molecular characterization of Acanthamoeba and Balamuthia mandrillaris from combination shower units in Costa Rica en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s00436-014-4083-6
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07


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