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Recombinant protein- and synthetic peptide-based immunoblot test for diagnosis of neurocysticercosis

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dc.contributor.author Noh, J.
dc.contributor.author Rodriguez, S.
dc.contributor.author Lee, Y.-M.
dc.contributor.author Handali, S.
dc.contributor.author Gonzalez, A.E.
dc.contributor.author Gilman, R.H.
dc.contributor.author Tsang, V.C.W.
dc.contributor.author Garcia, H.H.
dc.contributor.author Wilkins, P.P.
dc.date.accessioned 2020-06-10T18:12:15Z
dc.date.available 2020-06-10T18:12:15Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8074
dc.description.abstract One of the most well-characterized tests for diagnosing neurocysticercosis (NCC) is the enzyme-linked immunoelectrotransfer blot (EITB) assay developed at the CDC, which uses lentil lectin-bound glycoproteins (LLGP) extracted from Taenia solium cysticerci. Although the test is very reliable, the purification process for the LLGP antigens has been difficult to transfer to other laboratories because of the need for expensive equipment and technical expertise. To develop a simpler assay, we previously purified and cloned the diagnostic glycoproteins in the LLGP fraction. In this study, we evaluated three representative recombinant or synthetic antigens from the LLGP fraction, individually and in different combinations, using an immunoblot assay (recombinant EITB). Using a panel of 249 confirmed NCC-positive and 401 negative blood serum samples, the sensitivity of the recombinant EITB assay was determined to be 99% and the specificity was 99% for diagnosing NCC. We also tested a panel of 239 confirmed NCC-positive serum samples in Lima, Peru, and found similar results. Overall, our data show that the performance characteristics of the recombinant EITB assay are comparable to those of the LLGP-EITB assay. This new recombinant- and synthetic antigen-based assay is sustainable and can be easily transferred to other laboratories in the United States and throughout the world. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartof urn:issn:1098-660X
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Peru en_US
dc.subject Humans en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Animals en_US
dc.subject Antibodies, Helminth en_US
dc.subject Antigens, Helminth en_US
dc.subject Neurocysticercosis en_US
dc.subject Taenia solium en_US
dc.subject neurocysticercosis en_US
dc.subject human en_US
dc.subject Recombinant Proteins en_US
dc.subject immunoreactivity en_US
dc.subject priority journal en_US
dc.subject Taeniasis en_US
dc.subject controlled study en_US
dc.subject major clinical study en_US
dc.subject immunology en_US
dc.subject article en_US
dc.subject blood en_US
dc.subject taeniasis en_US
dc.subject animal en_US
dc.subject sensitivity and specificity en_US
dc.subject peptide en_US
dc.subject procedures en_US
dc.subject parasite antigen en_US
dc.subject polyacrylamide gel electrophoresis en_US
dc.subject diagnostic test accuracy study en_US
dc.subject feces analysis en_US
dc.subject recombinant protein en_US
dc.subject helminth antibody en_US
dc.subject enzyme linked immunoelectrotransfer blot en_US
dc.subject glycoprotein en_US
dc.subject immunoblotting en_US
dc.subject Peptides en_US
dc.subject protein purification en_US
dc.subject Glycoproteins en_US
dc.subject Immunoblotting en_US
dc.title Recombinant protein- and synthetic peptide-based immunoblot test for diagnosis of neurocysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.03260-13
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01

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