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Essential roles of zebrafish rtn4/Nogo paralogues in embryonic development

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dc.contributor.author Pinzón-Olejua, A.
dc.contributor.author Welte, C.
dc.contributor.author Abdesselem, H.
dc.contributor.author Málaga-Trillo, E.
dc.contributor.author Stuermer, C.A.O.
dc.date.accessioned 2020-06-10T18:12:16Z
dc.date.available 2020-06-10T18:12:16Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8082
dc.description.abstract Background: As a consequence of gene/genome duplication, the RTN4/Nogo gene has two counterparts in zebrafish: rtn4a and rtn4b. The shared presence of four specific amino acid motifs-M1 to M4-in the N-terminal region of mammalian RTN4, and zebrafish Rtn4b suggests that Rtn4b is the closest homologue of mammalian Nogo-A.Results: To explore their combined roles in zebrafish development, we characterized the expression patterns of rtn4a and rtn4b in a comparative manner and performed morpholino-mediated knockdowns. Although both genes were coexpressed in the neural tube and developing brain at early stages, they progressively acquired distinct expression domains such as the spinal cord (rtn4b) and somites (rtn4a). Downregulation of rtn4a and rtn4b caused severe brain abnormalities, with rtn4b knockdown severely affecting the spinal cord and leading to immobility. In addition, the retinotectal projection was severely affected in both morphants, as the retina and optic tectum appeared smaller and only few retinal axons reached the abnormally reduced tectal neuropil. The neuronal defects were more persistent in rtn4b morphants. Moreover, the latter often lacked pectoral fins and lower jaws and had malformed branchial arches. Notably, these defects led to larval death in rtn4b, but not in rtn4a morphants.Conclusions: In contrast to mammalian Nogo-A, its zebrafish homologues, rtn4a and particularly rtn4b, are essential for embryonic development and patterning of the nervous system. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartof urn:issn:1749-8104
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject Animals, Genetically Modified en_US
dc.subject adult en_US
dc.subject priority journal en_US
dc.subject controlled study en_US
dc.subject article en_US
dc.subject gene en_US
dc.subject immunohistochemistry en_US
dc.subject brain en_US
dc.subject embryo development en_US
dc.subject genetics en_US
dc.subject metabolism en_US
dc.subject animal tissue en_US
dc.subject nonhuman en_US
dc.subject animal en_US
dc.subject Down-Regulation en_US
dc.subject down regulation en_US
dc.subject physiology en_US
dc.subject Brain en_US
dc.subject reverse transcription polymerase chain reaction en_US
dc.subject Western blotting en_US
dc.subject nerve cell en_US
dc.subject Neurons en_US
dc.subject green fluorescent protein en_US
dc.subject zebra fish en_US
dc.subject in situ hybridization en_US
dc.subject gene silencing en_US
dc.subject molecular cloning en_US
dc.subject embryo en_US
dc.subject Zebrafish en_US
dc.subject confocal microscopy en_US
dc.subject Brain and spinal cord development en_US
dc.subject brain size en_US
dc.subject cell motility en_US
dc.subject Larval motility en_US
dc.subject Morpholino knockdown en_US
dc.subject morpholino oligonucleotide en_US
dc.subject myelin protein en_US
dc.subject Myelin Proteins en_US
dc.subject nerve fiber growth en_US
dc.subject Nogo en_US
dc.subject nogo gene en_US
dc.subject notochord en_US
dc.subject prenatal development en_US
dc.subject protein Nogo en_US
dc.subject Reticulon en_US
dc.subject reticulon 4 gene en_US
dc.subject retina en_US
dc.subject Retina en_US
dc.subject retina ganglion cell en_US
dc.subject retinotectal projection en_US
dc.subject rtn4 en_US
dc.subject Rtn4a protein, zebrafish en_US
dc.subject Rtn4b protein, zebrafish en_US
dc.subject superior colliculus en_US
dc.subject telencephalon en_US
dc.subject transgenic animal en_US
dc.subject zebrafish protein en_US
dc.subject Zebrafish Proteins en_US
dc.title Essential roles of zebrafish rtn4/Nogo paralogues in embryonic development en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/1749-8104-9-8
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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