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Use of a novel chagas urine nanoparticle test (chunap) for diagnosis of congenital chagas disease

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dc.contributor.author Castro-Sesquen, Yagahira E.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Galdos-Cardenas, Gerson
dc.contributor.author Ferrufino, Lisbeth
dc.contributor.author Sánchez, Gerardo
dc.contributor.author Valencia Ayala, Edward
dc.contributor.author Liotta, Lance
dc.contributor.author Bern, Caryn
dc.contributor.author Luchini, Alessandra
dc.date.accessioned 2020-06-10T18:12:17Z
dc.date.available 2020-06-10T18:12:17Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8093
dc.description.abstract BACKGROUND: Detection of congenital T. cruzi transmission is considered one of the pillars of control programs of Chagas disease. Congenital transmission accounts for 25% of new infections with an estimated 15,000 infected infants per year. Current programs to detect congenital Chagas disease in Latin America utilize microscopy early in life and serology after 6 months. These programs suffer from low sensitivity by microscopy and high loss to follow-up later in infancy. We developed a Chagas urine nanoparticle test (Chunap) to concentrate, preserve and detect T. cruzi antigens in urine for early, non-invasive diagnosis of congenital Chagas disease. METHODOLOGY/PRINCIPAL FINDINGS: This is a proof-of-concept study of Chunap for the early diagnosis of congenital Chagas disease. Poly N-isopropylacrylamide nano-particles functionalized with trypan blue were synthesized by precipitation polymerization and characterized with photon correlation spectroscopy. We evaluated the ability of the nanoparticles to capture, concentrate and preserve T. cruzi antigens. Urine samples from congenitally infected and uninfected infants were then concentrated using these nanoparticles. The antigens were eluted and detected by Western Blot using a monoclonal antibody against T. cruzi lipophosphoglycan. The nanoparticles concentrate T. cruzi antigens by 100 fold (western blot detection limit decreased from 50 ng/ml to 0.5 ng/ml). The sensitivity of Chunap in a single specimen at one month of age was 91.3% (21/23, 95% CI: 71.92%-98.68%), comparable to PCR in two specimens at 0 and 1 month (91.3%) and significantly higher than microscopy in two specimens (34.8%, 95% CI: 16.42%-57.26%). Chunap specificity was 96.5% (71/74 endemic, 12/12 non-endemic specimens). Particle-sequestered T. cruzi antigens were protected from trypsin digestion. CONCLUSION/SIGNIFICANCE: Chunap has the potential to be developed into a simple and sensitive test for the early diagnosis of congenital Chagas disease. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Acrylamides en_US
dc.subject Antigens, Protozoan/urine en_US
dc.subject Blotting, Western en_US
dc.subject Chagas Disease/congenital/diagnosis en_US
dc.subject Early Diagnosis en_US
dc.subject Female en_US
dc.subject Humans en_US
dc.subject Infant en_US
dc.subject Infant, Newborn en_US
dc.subject Latin America en_US
dc.subject Nanoparticles en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Trypanosoma cruzi/immunology/isolation & purification en_US
dc.title Use of a novel chagas urine nanoparticle test (chunap) for diagnosis of congenital chagas disease en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0003211
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735

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